...presented with a physical model of his large ribosomal subunit, he kept sliding his palm across the surface of... about Jacob’s initial reaction to this model on his blog. Or, for a description of how effective this...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

...guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University...Mary Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory...is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow him on Twitter...

...Blugene, Addgene's friendly mascot in a labcoat. And his ghost writers, of course! Amy Uddin and Emma Gottschall..., two of Addgene's lab technicians, gave Blugene his voice and wrote this lovely play-by-play of Addgene's...

... pitch,” of his/her project – kindly let the presenter know you are very interested in his/her work, and...approaches your poster, introduce yourself and ask about his/her background. This will give you a chance to determine...

...of Biosciences and Bioresources in Naples, Italy. His group works on spinal muscular atrophy, a disease...neurodegenerative effects of smn1 disruption. As part of his work in C. elegans, Dr. Di Schiavi will use Addgene...

...bag check was definitely worth it! Hearing about his passion for global health and the various initiatives...fellow Rhodobacter enthusiast, Tim also hosted me at his University of Wisconsin lab for a summer during my...

...tools are great for many applications, Verkhusha and his lab at Albert Einstein College of Medicine in New...that operate within the 750 to 800 nm range, and his team is also looking in the direction of biosensors...

...guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory...

...embraces open science, self-publishing directly on his website (and often posting updates on social media... vision of passive impact,” he says, noting that his team regularly shares troubleshooting tips and works...works with other labs to help them adopt the tools his group develops. And though it took a lot of work ...

...guest blogger Erik L. Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...the Department of Anatomy and Structural Biology. His interests include the quality control of secretory...

...and Lindsey M. Costantini! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...the Department of Anatomy and Structural Biology. His interests include the quality control of secretory.... Pubmed Central PMCID: PMC3079558. 8. Suzuki, Takahisa, et al. "Development of cysteine-free fluorescent...

... because of studies on OCRL1 already underway in his lab. The gene appears to play a role in many cell...Xeroderma Pigmentosum Hendrik De Buhr (top right) and his colleagues at the Wistar Institute in Philadelphia...

...deliverables. It does not mean a manager must allow his team to work mostly at home. Having a high degree...work place as described by Sebastiano Massaro in his Nature Biotechnology article “Managing knowledge-...

...know who participated was surprised to find that his friends and family finally understood why he cared...on some distant island. He got a little money for his trouble, too. 7)   Reach out to science writers in...

...out yet?) your research. Derek P. Simon received his PhD in Cellular and Molecular Biology from the University...Mary Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory...is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow him on Twitter...reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting an electrode and...technology is improving and may one day reach the sophistication of mouse transgenics. Everything that has been...

..., too! Bluegene is eager to decorate the lab but his short arms are causing him some difficulty… To give...

... pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available through Addgene. We have...Octet, Pall ForteBio). Tips for using pMal-T-Avi-His/BirA to add biotin to your protein of interest Grow...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins... the fusion protein includes an AviTag and a 6X HisTag for affinity purification (see plasmid map and ...dramatically increased accessibility of the AviTag and HisTag, which were “buried” in a few of our proteins that...proteins - but it would remove both the AviTag and HisTag. We were surprised that we couldn’t find an all-in-one...

...University studying mammalian immunology, and in his free time, he is pursuing a plant engineering project... cell culture and transgenesis he wants to share his knowledge with the community by teaching a DIY plant...

...jump into industry.” In his article On Leaving Academe, Terran Lane is asked by his colleagues and friends...

...tRNA ( 68307 ) at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein...