We narrowed to 263 results for: his-tag

...integrins, and more coming soon) as well as epitope tags like His, Flag, and Biotin. The antibodies developed ...%}{% end_module_attribute %}{% module_attribute "tag" is_json="true" %}{% raw %}"module"{% endraw %}{%...Binning at Moffitt Cancer Center in Tampa, Florida. His projects focus on using yeast display, structural...coupled with iterative rounds of selection and mutagenesis to refine antibody performance. If you are interested...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR. Current Protocols in Molecular Biology...

...additionally contain various combinations of protein tags (His, Avi, and GFP) as well as protease sites to facilitate...researcher to quickly generate N- and C-terminal epitope tag fusions to a protein of interest. The plasmids used...folding and/or protease sites to remove the epitope tags. Different plasmids in the kit can be used to purify...2015). He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison...conveniently performed through the N-terminal eGFP tag carried on all constructs. The Cell-Free Protein ...

...member of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing...the creation of innovative technologies. To date his group has develop several popular CRISPR-based tools..., Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression...We have found that SAM (Konermann et al., 2014), Suntag (Tanenbaum et al., 2014, Gilber et al, 2014), and...activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less about off targets Unlike...

...these self-labeling proteins are SNAP-tag® and HaloTag®. These tags consist of a genetically encoded enzyme...several advantages to using these self-labeling proteins over traditional fluorescent protein tags, such...endogenous target, and thus no genetically encoded tag is required. These dyes can also be attached to an...ligands have more flexibility due to the affinity tag they typically bind to. Stability of fluorescence...experimental labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression...fluorescent experiments, such as using fluorescently tagged ligands or intermolecular FRET for ligand-receptor...should possess high specificity to ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET...

...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable... be in academia to make change.  He talked about his own unique way of funding basic research at NEB and... of the Novartis industry postdoctoral program.  His strongest comments were about the astounding lack...alternate training needs to be provided to take advantage of all the possible science careers. She advocated...

...fusion protein consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest...precisely spatio-temporally controlled as a key advantage over other inducible enzyme technologies. She ...

...Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells...with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell...You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...collection (Link opens in a new window) of fluorescently-tagged human induced pluripotent stem cell (hiPSC) lines...researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell...TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) 114413 MYL7-mEGFP AICSDP-46 mEGFP MLC-2a Sarcomeric...

...Plasmid #68307) ) at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein...Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP...68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68300 MBP-MEK1... GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ ...Plasmid 68283 SepOTSβ Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1 XX Amp) Strain...Pooled Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306...BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka...

... Dr. Deisseroth has also generously made many of his optogenetic constructs available through the vector... at the University of North Carolina) that offer his constructs. Importantly, control viruses (not expressing...guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University...Mary Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory...is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow him on Twitter...and for good reason. For the first time in the history of neuroscience research we have a technology that...promoter of the cre recombinase while still taking advantage of the robust expression via the promoter in the...

...%}{% end_module_attribute %}{% module_attribute "tag" is_json="true" %}{% raw %}"module"{% endraw %}{%...Neural Dynamics and HHMI’s Janelia Research Campus. His research focuses on developing biosensors and tools...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...

...embraces open science, self-publishing directly on his website (and often posting updates on social media... vision of passive impact,” he says, noting that his team regularly shares troubleshooting tips and works...works with other labs to help them adopt the tools his group develops. And though it took a lot of work ...  Figure 1: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT magnet...effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable one-component magnetoresponsive... AsLOV2. After several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants...significant gains! After a few more rounds of mutagenesis, they eventually found a version of AsLOV2 with...

...guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University...Mary Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory...is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow him on Twitter...several groups [4, 5, 6]. These have the obvious advantage of not requiring patch cable connection to the...

...presented with a physical model of his large ribosomal subunit, he kept sliding his palm across the surface of... about Jacob’s initial reaction to this model on his blog. Or, for a description of how effective this... of proteins using 3D printing technology. The tagline for our company is “…where molecules become real...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

...can be found in Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of...plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins...Collection Botman-Teusink Yeast FP Collection FP Tagging FP Overexpression Resources This comprehensive ...allows for constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902...). Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-...

... Cloning of basic parts (promoters, UTRs, genes, tags, etc.) into MoClo-compatible vectors. Level 1: Assembly...this cloning technique! Many thanks to Rob Hurt for his help on this piece. Rob is postdoc in Peter Schultz...

...affinity for calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use ... have the option to indicate separate promoters, tags, or cloning information for each insert.  Encodes...insert into it. Empty backbones often contain epitope tags or fusion proteins that are appended to new inserts... indicate the gene cloned into your plasmid, any tags or fusion proteins contained in the plasmid, any...Use the drop-down menu to indicate whether the tag is on the N or C terminus and whether it was present ...

...guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory...Growth of new livers in culture could resolve this shortage." What is the knowledge gap you plan to address...

... mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5)...pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Addgene Resources Blog: Synthesized...

...epitope-targeting antibody that is conjugated to a biotin tag but not a fluorophore. In a separate step, cells ...interest. Indirect labeling is achieved through protein-tag or protein-protein interactions, like biotin-streptavidin... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...target marker, allowing for its detection. The advantage of this system is that streptavidin conjugates...the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility for panel...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

...promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which can...) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...