...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Reagents 1X PBS Microcentrifuge...staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...labeling cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody...need to be optimized for different cells, target proteins, etc. Sharing speeds science. We believe that ... Before Starting Refer to the manufacturer's instructions for additional information specific to your ...Paraformaldehyde fixation works well for many target proteins, it may not be the best fixation method for all...Remove the paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of...

... (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to ... packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized pool...

... control AAV collection, including fluorescent proteins, spaghetti monster, and more....Browse our control AAV that express fluorescent proteins under general promoters. These AAV can be used... Constitutive 2, 5, 8, 9, rg*, PHP.eB Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive...Dlx dTomato Cre dependent 1, 2, 5, 9, rg* Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH Syn EGFP Cre dependent...

...middle is hundreds, and the third is tens. Therefore, 1000 µL would read as 100 from top to bottom (as shown...Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top, which is used...tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring right below...come in standard sizes: P2, P10, P20, P200, and P1000. The table shows the pipette with the appropriate...This image shows the P2, P10, P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume... 2 to 20 µL P100 10 to 100 µL P200 20 to 200 µL P1000 100 to 1,000 µL Each pipette should be used with... dispense the correct amount of liquid reagent. P1000: The first red digit is thousands of µL, the middle...

...comprehensive human transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two ...scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive...ID PI Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor...

...commonly used adenovirus terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description...beta-gal Shuttle Test plasmid that contains β-gal and GFP; contains ∼10Kb genomic DNA stuffer Vogelstein...Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain modular inserts for seamless assembly...Collection of adenovirus shuttle vectors and strains Viral...Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids...database for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1...recombining shuttle plasmids; for use with large inserts Vogelstein 179201 pAd5-B6 ∆E3-GFP Adenoviral A ...

...pipettes Ice bucket to hold antibiotic Antibiotic at 1000 x concentration disolved in the appropriate liquid...use. Negative Result 1: Both Strains Grow Assuming the appropriate strains were streaked on the appropriate...your strains are no longer viable. You can check for this possibility by streaking out both strains on ...antibiotic to the molten gel mix, you should create a 1000x stock solution. For example: If you’ll be preparing...slightly. Even so, you should always use thermally insulated gloves when removing anything from the autoclave...appropriate plates, then if both strains grow, it’s possible that: The antibiotic broke down. You forgot to...concentration for selection. Negative Result 2: Neither Strains Grows If neither strain grows, it's possible that...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel pipette...titers were similar to those reported by these institutions. This protocol is for a 96-well plate with 20...run: 1.5 h Data analysis: 30 min Equipment qPCR instrument Heating plate Pipettors 1–10 µL single channel...Pro-Tips Use a "Universal" SYBR master mix which contains a high-quality DNA polymerase and a blend of dTTP...95 uL 20X 200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution 4 20 uL Dil. 3 80 uL 5X 5000X Dilution ...the final volume (mix with >50 uL if your well contains 100 uL) Use a multichannel pipette to load the...tube. Run the following protocol in your qPCR instrument using SYBR detection: 98 °C 3 min / 98 °C 15 ...

... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

...inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both... edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor DNA at “...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...

...Plasmids 101: E. coli strains for protein expression Plasmids 101: Common Lab E. coli Strains Why Bacteria? E...Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors with N and...Expression: contains mEos2 with an N-terminal His tag. Photoactivable fluorescent proteins like this one... 3OC6HSL (Quorum sensing molecule) Cynthia Collins Contains luciferase inducible by the quorum sensing...green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids with various combinations...factor bind to a particular sequence? Do these two proteins interact? What happens when I overexpress this...From recombineering to CRISPR , this collection contains a variety of tools to help you harness bacterial...

...Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under the control ...Cre dependent 5, 9 Tian Calcium Sensor: Twitch2B 100040 pAAV.hSyn1.Twitch2B.WPRE.SV40 Syn Twitch2B none...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel pipette...modified for other targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit ...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: July 7, ...well untransduced (add 150 µL of DMEM complete instead of a viral dilution). Remember, this is an additional... to the GeneJet Genomic DNA Purification Kit instructions. Determine the concentration of each sample ... To ensure that the droplets are not disrupted, insert the pipette tips directly in the center of the ... To ensure that the droplets are not disrupted, insert the pipette tips and gently touch the bottom of...

...GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's L. Miguel...Miguel Martins 14124 pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2...Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's L. Miguel Martins 15940 pcDNA3-HtrA2...Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's L. Miguel Martins 16155 pET20b-...His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-...

...inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both... edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor DNA at “...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...

... or more from the same tube, but unlike using a p1000 tip it will give you more control of where you’re...LiCl selectively precipitates RNA versus DNA or proteins. Add the correct amount of 7.5 M LiCl solution...downstream applications. To improve yield of RNA, instead of incubating at -20°C for 1 hour, you can try ...

...purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol ...bring up the volume with formulation buffer. Use a P1000 to the bottom of the filter and pipette up/down ...of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image adapted...