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We narrowed to 74 results for: mcherry expressing plasmid

Showing: 61 - 74 of 74 results
  1. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-CaMKIIa-mCherry CamKIIa mCherry Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738...preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and ...Activator C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein...107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible...rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA minBG iCre expression iCre Ting 199775 AiP13778 - pAAV-AiE0450h-minBG-iCre...
  2. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped...activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter...
  3. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express gene ...Vector Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...
  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...MiCy-N1 - Mammalian Expression MiCy-C1 - Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top...Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 ...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ...Mammalian Expression pET-MCN-10His-mStayGold (E138D) - Bacterial Expression pJRA110 - Plant Expression mStayGold...
  5. Recombinases AAV Preps

    Type
    Collection
    ...which can be used to control gene expression. See our Cre-lox Plasmids page for more information about ..., 9 James M. Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Karl Deisseroth... 9 James M. Wilson 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato... rg* Ian Wickersham 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Karl Deisseroth...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....
  6. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes reported...Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes...mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was...
  7. AAV Molecular Tools

    Type
    Collection
    ...241241 GfaABC1D-Lck-mCherry-ibARK GfaABC1D-driven, constitutive Expression of mCherry-tagged, plasma membrane-tethered...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118...constitutive Expression of SpCas9. 8 Hetian Lei 104588 pAAV-EFS-SpCas9 EFS-driven, constitutive Expression of Myc-tagged...transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must...99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA). 2 Viviana Gradinaru...pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive...positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent...
  8. Control AAV Preps

    Type
    Collection
    ...Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive 5 Edward Boyden 59133 pOTTC589...2 Marcella Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB...PHP.eB Karl Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Karl ...Karl Deisseroth 114472 pAAV-hSyn-mCherry hSyn mCherry Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth 117382...Zhang 20299 pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA EF1a mCherry Cre dependent 9 Karl Deisseroth 27056...112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent 1, 2, 5, ...8, 9, rg* Bryan Roth 50459 pAAV-hSyn-DIO-mCherry hSyn mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth...
  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...guide construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...reporter) pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its...addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling...shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-...activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654 and 159655...159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow conversion...
  10. Optogenetics Guide

    Type
    Guide
    ... experiment. A channelrhodopsin fused to mCherry is expressed in neurons (red dots). When exposed to light...exhaustive — check out our Optogenetics Plasmid Collection to find plasmids for these and other tools for your...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...Explore Addgene's Optogenetics Plasmid Collection to find the plasmids you need. Looking for viral preps...Learn about using Optogenetics plasmids in your lab, including microbial opsins and optical switch systems...practical tips , explore Addgene's Optogenetics Plasmid Collection , or read on to learn more about these...
  11. Fluorescent Proteins: FRET

    Type
    Collection
    ...backbones and control plasmids. Donor Acceptor λ Dex QY D λ Aem ε A QY A R 0 J(λ) Plasmids mTagBFP sfGFP 399...mCitrine-pBAD EGFP mCherry 488 0.60 610 72,000 0.22 5.3 1.9 mEGFP-N1 , mCherry2-N1 , pcDNA3.1 CMV mCherry-eGFP BGH.... Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection to find related tools...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...FRET on our blog and explore the tables below for plasmids and information to help plan your next FRET experiment...,800 0.62 5.7 2.9 mVenus N1 , mKO2-N1 mOrange2 mCherry 549 0.60 610 72,000 0.22 6.1 4.4 mOrange2-N1 , ...
  12. Genetic Code Expansion

    Type
    Collection
    ...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic... Plasmid Collections Genetic Code Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the...protein translation machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS ... ncAAs. This plasmid often carries multiple tRNA copies for efficiency. A target plasmid containing the...are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that contain aminoacyl-tRNA synthetases...
  13. Tetracycline Inducible Expression

    Type
    Collection
    ...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...Promoters Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids References Das, A. T., Tenenbaum...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR ...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...
  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Optogenetics Plasmids Viral Service: AAV Biosensors Luciferase Plasmids Promega Plasmid Collection Explore...NADH/NAD+ Cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants In Vivo NADH... of S. cerevisiae MOSAIC Kit includes 35 plasmids expressing a variety of fluorescent sensors to monitor...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...
Showing: 61 - 74 of 74 results