Skip to main content

We narrowed to 73 results for: mcherry expressing plasmid

Showing: 61 - 73 of 73 results
  1. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes reported...Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes...mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was...
  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...6B) - Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...MiCy-N1 - Mammalian Expression MiCy-C1 - Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top...Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 ...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ...
  3. Lentivirus Plasmids

    Type
    Collection
    ... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ... VSV-G-expressing envelope plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier...Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA? Browse our...catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description PI 8453...destination plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression of EGFP... 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato version...Dmitri Wiederschain 36084 pLV-mCherry 3rd CMV driven expression of mCherry fusion. See 36083 and 36086 ...
  4. Fluorescent Proteins: FRET

    Type
    Collection
    ...mCitrine-pBAD EGFP mCherry 488 0.60 610 72,000 0.22 5.3 1.9 mEGFP-N1 , mCherry2-N1 , pcDNA3.1 CMV mCherry-eGFP BGH...backbones and control plasmids. Donor Acceptor λ Dex QY D λ Aem ε A QY A R 0 J(λ) Plasmids mTagBFP sfGFP 399.... Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection to find related tools..., mKO2-N1 mOrange2 mCherry 549 0.60 610 72,000 0.22 6.1 4.4 mOrange2-N1 , mCherry2-N1 miRFP670 miRFP720...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...FRET on our blog and explore the tables below for plasmids and information to help plan your next FRET experiment...
  5. Optogenetics Plasmid Collection

    Type
    Collection
    ... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection Microbial...Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources Optogenetics plasmids encode proteins ...These proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to control...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...
  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...guide construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...reporter) pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its...addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling...shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-...activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654 and 159655...159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow conversion...
  7. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled...with or without GFP and mCherry controls. Do you have suggestions for other plasmids that should be added...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...
  8. Chemogenetics Plasmids

    Type
    Collection
    ...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection Chemogenetics plasmids encode genetically engineered...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for chemogenetic experiments, including popular DREADDs and PSAMs. Plasmid... Plasmid Collections ...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors...
  9. Antibody Plasmid Collection

    Type
    Collection
    ... Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made...created by cloning antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast... Guide ! Cloning and Expressing Antibodies The following table highlights plasmid collections that can...can be used to create and express plasmid-based antibodies. Description Article PI One-step assembly of... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...
  10. Optogenetics Guide

    Type
    Guide
    ...Optogenetics Plasmid Collection . An optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...Explore Addgene's Optogenetics Plasmid Collection to find the plasmids you need. Looking for viral preps...domain (iLID plasmid), while its natural binding partner SspB is included in a separate plasmid; Blue light...photoreceptor for light-controlled gene expression. These plasmids rely on the engineered two-component ...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...indicators or voltage sensors, visit our Biosensors Plasmid Collection . Microbial Opsins Opsins are light-...
  11. Tetracycline Inducible Expression

    Type
    Collection
    ...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR ...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...
  12. Genetic Code Expansion

    Type
    Collection
    ...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic... Plasmid Collections Genetic Code Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the...protein translation machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS ... ncAAs. This plasmid often carries multiple tRNA copies for efficiency. A target plasmid containing the...are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that contain aminoacyl-tRNA synthetases...
  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Optogenetics Plasmids Viral Service: AAV Biosensors Luciferase Plasmids Promega Plasmid Collection Explore...NADH/NAD+ Cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants In Vivo NADH... of S. cerevisiae MOSAIC Kit includes 35 plasmids expressing a variety of fluorescent sensors to monitor...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...
Showing: 61 - 73 of 73 results