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We narrowed to 397 results for: nam

Showing: 61 - 80 of 397 results

  1. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... in your database to the effect of `dev_name` and `science_name`. You'll need to decide given the technical...things tracked. Terminology and language are tricky Names for things will trip everyone up at some point, ..., decide early on what to do in a namespace collision. It may even be worth it to have extra columns in... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

  3. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...sequence or name. You can perform more in-depth analyses on each sample by clicking on the sample name or on...and provide basic information such as your sample names and guide sequences, and ICE will do the rest. There...shows a green checked circle in front of the sample name. If there was a minor error during processing, the...following parameters: Sample - The unique label name that you provided for each sample. Guide Target ...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...bright Recent series of enhanced monomeric NIR FPs named miRFPs (monomeric infrared FPs) (Matlashov et al...recommend using miRFPs and their enhanced versions named emiRFPs in your applications.  There is a whole ...distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra....  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome... An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... to control the cytoskeleton and cell membrane dynamics. First, a quick refresher.  The tools below use...microtubule-severing protein, Katanin is of course named for the samurai sword called a katana. Opto-katanin...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, and more, often...proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and many functions... the many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-...

  6. AAV for Neuronal Tracing

    Type
    Collection
    ...AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

  7. Chemogenetics AAV Preps

    Type
    Collection
    ... AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement...

  9. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We hope everyone... tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria...bacteria, fungi, viruses, and one microbe ornament even dyed with real microbial pigment. Check out the hashtag...hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...have different names, and the software can only extract gRNAs for genes using the name that is used by...condensed text file containing a full and a short name for each gRNA, as well as the suggested gRNA sequence...one solution is to identify all the alternative names for your gene of interest (that were not found by...Green Listed), and test if any of the alternative names works better. To achieve this you could e.g. copy...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...when the protein is present in a sample — just to name a few. Traditional sources of antibodies for research...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...BioRender.com. The original prime editing enzyme is named PE1 . PE1 and most other prime editors use the Moloney...

  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...Visualization of DA release dynamics during locomotion in freely moving mice Record DA dynamics of optogenetically... single neuron in vivo in Drosophila Detect DA dynamics in response to a threatening looming stimulus ...et al. "Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...within an organism.  So how did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the...and François Wollman and became the widely adopted name for these elements. At the time, the use of episome...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected...store, QC, curate, and distribute them all in the name of making it a little bit easier for scientists ...

  15. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... intensity and wavelength can be used to study dynamic processes. Conversely, varied input patterns may... activity persisted in the inactive state. The dynamic range, defined as the difference between the lowest...They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements will...system. Applications of these systems The large dynamic range of these systems ensures that they can be...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize the structural...CoChR), and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue and green light-excitable and...than those of other channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked ...0.4ms from 21.4 ± 1.1 ms); this new variant was named ChrimsonR. Plasmids containing Chronos, Chrimson... overexpression or knock-down, where signaling dynamics are influenced by the relatively long life-cycle...assembly of plant transformation constructs. As the name suggests, GreenGate is based on the Golden Gate ...

  17. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...handy and make sure your name tag is always visible. Some people learn names by seeing them. It is OK ...level. Right away you have something in common. Namely, the scientific question you are interested in ...OK to ask someone to see their name tag if you are one of those people. Meet people in sessions by sitting...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9...

  19. Deep Dive: qPCR

    Type
    Blog Post
    ...probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are other chemistries available...online tool that uses the  amplicon sequence and thermodynamic and experimental parameters (neighbor stacking...temperature range) to predict PCR melting curves and dynamic melting profiles (Dwight, 2011).  Reference Genes...prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web ...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...infection and to monitor their abundance and clonal dynamics over time using next generation sequencing (NGS...the ClonTracer library and monitored population dynamics after treatment with EGFR inhibitor, erlotinib...using the ClonTracer library to monitor clonal dynamics following drug treatment in cell line and primary... 1. Hyo-eun, C. Bhang, et al. "Studying clonal dynamics in response to cancer therapy using high-complexity...
Showing: 61 - 80 of 397 results