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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 288 results for: nif

Showing: 61 - 80 of 288 results

  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...have been identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus),... 562 61 Luciferin ATP No Sea pansy (Renilla reniformis) Natural 481 36 Coelenterazine None No Click...

  3. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...reverse screens, except that they begin with a uniform population possessing a specific mutation or phenotype... the cellular pathway of interest conveys no significant survival advantage, such as a non-essential mechanism...

  4. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...

  6. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... to detect mRNA from 10 bacteria of clinical significance and four clinically relevant host biomarkers... Jennifer Tsang...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    ...amounts of access or feedback. One of the most significant shifts in scientific communication is the rising...research materials available to the community significantly sooner. Plus, authors can include the Addgene...

  9. A Look at Addgene's QC Process

    Type
    Blog Post
    ...elements, and nucleotide indels or mismatches in significant plasmid elements. Which differences are considered...This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley...

  10. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...compatibility. This alternative PAM requirement adds a significant amount of flexibility compared to a strict single-compatibility...increases the flexibility of gRNA placement significantly over SpCas9 and more so than Cas9s with an NG...

  13. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...necessary step during production), there is a significant risk for aerosol contamination. As most centrifugation...sharps procedures for your organization will significantly reduce the risk of an accident (the most common...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ... proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally...10.1016/j.celrep.2020.107804 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...system developed to track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a .../genetics/iyab006       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...disease, and HIV. A notable landmark study showed significant clinical efficacy against a form of blindness... of LCA and mutations in RPE65 (n=3), showed significant improvements in vision over a period of 1 to ...

  19. CRISPR in the Clinic

    Type
    Blog Post
    ...therapies that are currently approved or have made significant progress in clinical trials.     Figure...delivery choice. In sum, CRISPR therapies have made significant clinical strides since we first wrote this blog...

  20. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... a freshly collected lentiviral prep may be significantly higher than the same prep that has been stored...PMCID: PMC1534021. 3. Zhang, Bing, et al. "The significance of controlled conditions in lentiviral vector...
Showing: 61 - 80 of 288 results