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  1. The Advances Behind The World's First Personalized CRISPR Treatment

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    ..., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang...., Dahl, G. A., Correia, B. E., … Kleinstiver, B. P. (2025). Custom CRISPR-Cas9 PAM variants via scalable...Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting with near-... Emily P. Bentley...
  2. Antibodies 101: Choosing the Right Isotype

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    ....org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding...In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https:...
  3. Hot Plasmids: February 2026

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    ... here! Ding, Y., He, W., Wang, K., Xue, F., … Xu, P. (2026). A highly photostable monomeric red fluorescent...here!   Secure self-storage in the vault By Emily P. Bentley Like a time capsule you bury in your yard...bacterial strain with a single stop codon By Emily P. Bentley That's right: just one! Simple enough to ...tuned for their viral preps coming soon! DeWeirdt, P. C., et al. (2022). Accounting for small variations...
  4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS...https://doi.org/10.1038/nbt1153 Odom GL, Gregorevic P, Allen JM, Chamberlain JS (2011) Gene Therapy of mdx...://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco ....201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, ...
  5. Viral Vectors 101: Chemogenetics

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    ... and Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First .../doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X,...content/364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard...
  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000 colonies...human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria...Enterobacter aerogenes, Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown...
  7. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation of Prokaryotic...Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M,...Merten O-W, Blouin V, François A, Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules...
  8. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide specificities of CRISPR-Cas ...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF...
  9. CRISPR 101: Cytosine Transversion Editors

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    ... References Chen, L., Park, J. E., Paa, P., Rajakumar, P. D., Prekop, H.-T., Chew, Y. T., Manivannan..., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram, A., & Liu, D. R. (2018). Improving cytidine....4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald, J., & ... Emily P. Bentley...
  10. A Needle in a Base-Stack: Cas9 Structural Biology

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    ... Other Cases Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural biology of CRISPR–Cas...doi.org/10.1038/s41579-022-00739-4 References Bravo, J. P. K., Liu, M.-S., Hibshman, G. N., Dangerfield, T. ...annurev-biophys-062215-010822 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani....201796941 Pattanayak, V., Lin, S., Guilinger, J. P., Ma, E., Doudna, J. A., & Liu, D. R. (2013). High-throughput...  Emily P. Bentley...
  11. Build Your CRISPR Vocabulary

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    ...! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural biology of CRISPR–Cas... Emily P. Bentley...
  12. Tag Your Favorite Yeast Genes with Ease

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    ...NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. Longtine...Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note:...
  13. Magnetic Control of Proteins: More than a Dream

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    ...A. M., Grippo, R. M., Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel, M. K., Deppmann..., M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field...
  14. Tips for Screening with Yeast Two Hybrid Systems

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    ...Chen, Y.C., Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid ...668. Pubmed PMID: 20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system ...20719741. Pubmed Central PMCID: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino...
  15. Light Sheet Fluorescence Microscopy

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    ...point the direction of the specimen, A= anterior, P= posterior, D= dorsal, V= ventral Developments in ...et al. (2007). Nature Methods, 4, 331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483....Development, 105.61-74. Pubmed. J. Sharpe, U. Ahlgren, P. Perry, Hill B, Ross A, J. Hecksher-Sorensen, R. Baldock...
  16. Lentivirus ddPCR Titration

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    ...Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for...
  17. Antibodies 101: Antibody Engineering and Directed Evolution

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    ...https://doi.org/10.3390/antib8040055 Holliger, P., & Hudson, P. J. (2005). Engineered antibody fragments and...References and Resources References Chan, A. C., & Carter, P. J. (2010). Therapeutic antibodies for autoimmunity...394. https://doi.org/10.1038/nrg3927 Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021)....
  18. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...scale.     References Bezanilla M, Perroud P ‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella...Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T...
Showing: 61 - 80 of 252 results