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We narrowed to 242 results for: p

Showing: 61 - 80 of 242 results
  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post
    ...colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000 colonies...human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria...Enterobacter aerogenes, Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown...
  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... and Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First .../doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X,...content/364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard...
  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide specificities of CRISPR-Cas ...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF...
  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... References Chen, L., Park, J. E., Paa, P., Rajakumar, P. D., Prekop, H.-T., Chew, Y. T., Manivannan..., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram, A., & Liu, D. R. (2018). Improving cytidine....4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald, J., & ... Emily P. Bentley...
  5. A Needle in a Base-Stack: Cas9 Structural Biology

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    ... Other Cases Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural biology of CRISPR–Cas...doi.org/10.1038/s41579-022-00739-4 References Bravo, J. P. K., Liu, M.-S., Hibshman, G. N., Dangerfield, T. ...annurev-biophys-062215-010822 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani....201796941 Pattanayak, V., Lin, S., Guilinger, J. P., Ma, E., Doudna, J. A., & Liu, D. R. (2013). High-throughput...  Emily P. Bentley...
  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural biology of CRISPR–Cas... Emily P. Bentley...
  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. Longtine...Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note:...
  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...A. M., Grippo, R. M., Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel, M. K., Deppmann..., M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field...
  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Chen, Y.C., Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid ...668. Pubmed PMID: 20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system ...20719741. Pubmed Central PMCID: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino...
  10. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...point the direction of the specimen, A= anterior, P= posterior, D= dorsal, V= ventral Developments in ...et al. (2007). Nature Methods, 4, 331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483....Development, 105.61-74. Pubmed. J. Sharpe, U. Ahlgren, P. Perry, Hill B, Ross A, J. Hecksher-Sorensen, R. Baldock...
  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post
    ...scale.     References Bezanilla M, Perroud P ‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella...Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T...
  12. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...https://doi.org/10.3390/antib8040055 Holliger, P., & Hudson, P. J. (2005). Engineered antibody fragments and...References and Resources References Chan, A. C., & Carter, P. J. (2010). Therapeutic antibodies for autoimmunity...394. https://doi.org/10.1038/nrg3927 Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021)....
  13. Viral Production

    Type
    Collection
    ... element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization...
  14. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...J. E., Dunn, B. M., Speicher, D. W., & Wingfield, P. T. (Eds.). (2001). Current Protocols in Protein Science...Maltose-Binding Protein as a Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols...
  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ....2019.01.003  Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). An In Vivo Covalent TMP-Tag...better-dyeing-through-chemistry-small-molecule-fluorophores  Los, G. V., Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N....M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins, A., Klaubert, D...10.1021/cb800025k  Miller, L. W., Cai, Y., Sheetz, M. P., & Cornish, V. W. (2005). In vivo protein labeling...
  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for...
  17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...PubMed Central PMCID: PMC2593125. 2. Buchholz, F, P O Angrand, and A. Francis Stewart. 1998. “Improved...Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase of the Saccharomyces...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...
  18. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR...References Chew WL, Tabebordbar M, Cheng JKW, Mali P, Wu EY, Ng AHM, Zhu K, Wagers AJ, Church GM (2016)...
Showing: 61 - 80 of 242 results