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Synthetic origins of replication, or ORIs, from the Chappell Lab have improved plasmid compatibility and copy number control compared to natural ORIs.

Find tips for using the chromatogram to analyze your Sanger DNA sequencing results.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Find out what were Addgene's most popular blog posts in 2020!

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Best practices for performing sequence analysis of a plasmid

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

Protocols for plasmid cloning by PCR.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Addgene's new viral service provides ready-to-use viral preps for your research.

Get started with Gibson Assembly Cloning!

Protocol for Ligation Independent Cloning

Learn about SnapGene's feature database and how it can be used to identify sequence elements in your plasmids.

Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

We recently attended the Society for Neuroscience conference in Washington DC. Check out some of what we learned about this exciting field.

Learn more about Addgene's NGS verification process for pooled libraries using real-life amplication data from the Brunello gRNA library.

Addgene Executive Director Joanne Kamen's discusses the new Viral Service, it's sponsors, and how it helps scientists accelerate their research.