We narrowed to 1,055 results for: sta

...H840A nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations...protection factor La to PEmax to enhance pegRNA stability epegRNA — additional protection added to 3’ tail...

...Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive...Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene constructs...

...two formats: pretransformed in bacteria as an agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25...window) is a 19.1 kDa and exceptionally bright and stable luciferase that enables highly sensitive detection...

...interaction with negative regulators, increase its stability and DNA binding activity, and allow it to bind...regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also known as PMAIP1...

...fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all contribute to assess...Hepworth, D., Liu, S., Noell, S., Piotrowski, M., Stanton, R., Zhang, H., Corallino, S., Faedo, A., Insidioso...

...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition ...some restrictions (for example, the target must start with a T), but they still have potential targetable...

...scientific research ecosystem, not just in the United States but around the world. As an organization that directly...research infrastructure that has taken decades to establish.These funding decisions will have far-reaching consequences...progress depends on our collective response. Addgene stands ready to collaborate with partners across the research...will directly shape our response initiatives as we stand with the scientific community. Let us know by emailing...

...choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things...resources for hiring research staff. And for a more indepth resource for starting your academic lab, download...a PI without a personal assistant has to deal with. My inbox currently stands at about 65,000 messages...for advice on staffing your lab. Let us know if you have any relevant tips for starting a lab. Or share...effective working environment? Tom Ellis was offered a start-up package that included a postdoc and a PhD student...important to me to be with them in the lab when they started, so that we could discuss our projects and ideas...

...was GeoCas9 discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new...active at high temperatures Figure 1: Thermostability of SpCas9 vs GeoCas9. SpCas9 or GeoCas9 RNPs...GeoCas9’s editing activity in HEK293’s. GeoCas9 stability in human plasma The last trick GeoCas9 has up ...Harrington et al suspected GeoCas9 might have higher stability since prior research had shown that a cocaine ...cocaine esterase engineered to have increased thermostability also had a longer lifetime in human blood. To ...and cleavage product analyzed on a gel. SpCas9 is stable when incubated in reaction buffer alone, but has...lower than optimal temperatures. GeoCas9’s thermostability could also be used with in vitro molecular ...

...involved, it can be difficult for scientists to understand how GPCR activation leads to downstream effects... the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2...resulting in a decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio...pharmaceutical drugs, there is still only a partial understanding of how GPCRs signal through G protein signal...provides scientists with a toolkit to gain an understanding of the signaling through the transducerome from...as well as the activation by drugs to further understand their mechanism. These plasmids are available... the transducerome, to gain a more complete understanding of a GPCR mediated response.  Acknowledgements...

...Biomedical research, for instance, contributes heavily to medical practice, and understanding the scientific process...can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more involved... maintenance Administrative assistant: assists PI with admin duties Staff scientist: similar role to a...professors, and other mentors, and four years after I started college, I will be entering a PhD program to continue... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us... process can help us understand how new therapies can be developed and applied. The current pandemic serves...the lab Official title is typically Professor, Assistant Professor, Associate Professor Sometimes teaches...

... you. Statistics – The basics Statistical analysis is what determines if there is statistical significance... several core factors of statistics relevant for many downstream statistical tests.   Independent variables...are statistically different. They are classic parametric tests and one of the simplest statistical tests...described above. Violated assumptions and statistics Statistical power, the ability to correctly identify...is a better option. Stay tuned for our upcoming blog on non-parametric statistical tests! Adding it all...your friendly neighborhood biostatistician happy by making your statistical analysis plan when you design...Wissing, Dennis R., and Timm, Donna. Statistics for the nonstatistician: Part I. South Med J., 105(3): 126...

... creation of the United States Environmental Protection Agency, which started making regulations such ... the second in a series of posts on bringing sustainable practices in the lab. The first post focused ...learn about some creative approaches to lab sustainability, and find ways to further reduce our consumption...consumption beyond recycling.     Choosing more sustainable products Recycling is great for rerouting waste... grant agencies), we can shift toward a more sustainable one. The math on this is pretty simple: wash ...solutions and practices to meet higher environmental standards. Find some easy action items on our other article...

...location where the United States Postal Service picks up mail. NEB actually started this program in 1976! ..., flip the flaps of the box so the pre-paid postage stamp is on the outside of the container, and drop...off the box at any location where the United States Postal Service picks up mail. Recycle plastic packaging...more lab sustainability tips, check out our other articles in this series: Bringing Sustainable Practices...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...cost of regular waste services. Learn about how to start a glove recycling program at your institute! What...culture room for the wrappers and ship them back. Start a glove recycling program at your institute! Universities...

...can use the loss of resistance as a marker for successful cloning. In these instances, the cloning vector...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think...to maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once...pressure to keep your plasmid. Viva la (bacterial) resistance!  What are antibiotics? Antibiotics are generally...bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction between the two ...categories may be a bit of a gray area as some bacteriostatic reagents can kill bacteria when used at high...subunit; inhibits aminoacyl translocation Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide...

...recombination between the two FRT sites, resulting in the stable integration of the gene of interest into the genome... repetitive recombination leading to genomic instability and cell death. It is important to incorporate...

...zebrafish, mouse, rat, and human. Developed by the Stanley Qi Lab . CCTop - CRISPR/Cas9 target online predictor...Link opens in a new window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description...

...challenging for researchers using CRISPR, and that standardizing and improving the gRNA design process and rules...applications is necessary going forward. The standardization of CRISPR methods was a common thread at the...NIH Common Fund, emphasizes that we need more standardized methods for evaluating CRISPR technologies, ...basic or early pre-clinical research. However, standard terms and techniques are not enough to move the...including a detailed look at how cut-to-mutation distance affects editing efficiency. Optimizing Donor DNA...members of Jacob Corn’s lab describe how a better understanding of Cas9 biophysics can help enhance HDR. CRISPR...

...to compare the photostability of different fluorescent proteins? Measuring photostability Since it is clear... to quantify the photostability of fluorescent proteins. To determine photostability, an experiment is...Measuring photostability at realistic powers will provide better insight into the photostability in the ...Measuring photostability in living cells provides a much more realistic view of photostability. Selecting...This avoids substantial changes in excitation power and will help to keep the photostability of the probes...this series, we will discuss how to select a photostable fluorescent protein. Photobleaching is the irreversible...desirable to have fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins...

... Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It ...their inclusion here adds much needed context to understand every other problem. For non-scientists, Zimmer... ago. Teaching by example No examination of the state of science would be complete without the inclusion...to. The problems and challenges described in The State of Science have not changed in “COVID times,” but...next week.   A stepping stone When I discussed The State of Science with the five Addgene colleagues who ... learn everything about one specific topic, The State of Science could be a jumping off point to explore...