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We narrowed to 1,046 results for: sta

Showing: 61 - 80 of 1046 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...cells, are already resistant to this antibiotic due to stable integration of resistance genes in their genomes...selection agent. Pros: Stable (spectinomycin). Cons: Cost (spectinomycin) or stability (streptomycin), doesn...damage. Choosing an antibiotic resistance Choosing the best antibiotic resistance ultimately comes down to ...antibiotic and happy selecting!   Antibiotic Resistance Stability Time Saving?  Works for multiple organisms... M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin by stable expression of the Sh ble gene...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your...interest. But does it matter which antibiotic resistance gene you select? In this blog, we’ll break down...

  2. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...to compare the photostability of different fluorescent proteins? Measuring photostability Since it is clear... to quantify the photostability of fluorescent proteins. To determine photostability, an experiment is...Measuring photostability at realistic powers will provide better insight into the photostability in the ...Measuring photostability in living cells provides a much more realistic view of photostability. Selecting...This avoids substantial changes in excitation power and will help to keep the photostability of the probes...this series, we will discuss how to select a photostable fluorescent protein. Photobleaching is the irreversible...desirable to have fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins...

  3. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Though Cas9 is not a static molecule in any binding state, RNA loading stabilizes the closed conformation...pyogenes.   Figure 2:  Crystal structure of S. pyogenes Cas9 in the apo state (PDB ID 4CMP) with domains...the PAM is so flexible in this state that it doesn’t appear in the crystal structure (Figure 2) at all. ...Pro tip! X-ray crystallography cannot capture flexible or disordered regions, so crystal structures like...in this region. The crystal structure in Figure 3 shows the guide RNA stabilizing the DNA-binding protein...pyogenes Cas9 in the apo state (PDB ID 4CMP, top) compared to the guide RNA-bound state (PDB ID 4ZT0, bottom...engineering, and studying its structure can help us understand the parameters of CRISPR experimental design....

  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... camera, OR, monitor from that distance.  Build: We got a wheeled stand and mounted a monitor to it. It...Addgene’s Science Video Production Specialist, I’m constantly thinking about ways to improve our productions...Pivot Back in March 2020, just four months after I started with Addgene, I was about to have a check-in with...resources we have, and we developed a great plan to start consistently knocking out high-quality videos for...the window. So, much like SynBio, it was time to start our Design-Build-Test-Learn cycles. If you’re not...filming myself. While easiest from a production standpoint, it wasn’t ideal for our Lab Protocol videos....space to shoot was another hurdle. As we slowly started to realize that we were going to be out of the ...

  5. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ... Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It ...their inclusion here adds much needed context to understand every other problem. For non-scientists, Zimmer... ago. Teaching by example No examination of the state of science would be complete without the inclusion...to. The problems and challenges described in The State of Science have not changed in “COVID times,” but...next week.   A stepping stone When I discussed The State of Science with the five Addgene colleagues who ... learn everything about one specific topic, The State of Science could be a jumping off point to explore...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...number of participants and the standard academic calendar. So you want to start a journal club? Here are some...begin building a high level understanding across multiple teams or to stay abreast of current trends in...certainly had concerns that I may not have time to stay current or enjoy that discussion. Luckily, one needn't... edge science, researchers of all varieties must stay current with literature and emerging technologies...recurring meeting amongst scientists that aims to help stay current on new and emerging work by collaboratively... The first thing to do if you’ve thought about starting a journal club is to find a topic and seeing if...clear purpose and effective time management, for starters.  Now, you are ready for your first journal club...

  7. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...challenging for researchers using CRISPR, and that standardizing and improving the gRNA design process and rules...applications is necessary going forward. The standardization of CRISPR methods was a common thread at the...NIH Common Fund, emphasizes that we need more standardized methods for evaluating CRISPR technologies, ...basic or early pre-clinical research. However, standard terms and techniques are not enough to move the...including a detailed look at how cut-to-mutation distance affects editing efficiency. Optimizing Donor DNA...members of Jacob Corn’s lab describe how a better understanding of Cas9 biophysics can help enhance HDR. CRISPR...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...musician, then metastasize is touring. But you know what a touring musician needs? A backstage team. Meet ...progression, and metastasis. The microenvironment’s physiology is deeply connected to these 3 stages and contains...microenvironments have started to gather attention. By the 1960’s, scientists have begun to understand two main areas... seem to take center stage in cancer development, the microenvironment backstage play just as important...Meet the microenvironment, a tumor’s backstage team. Microenvironments are cellular regions with specialized...highly prone to affect it. While tumors may be the stars, there's plenty of action outside of tumor cells...them, making it even harder to cure cancer. To understand how cancer cells influence microenvironments ...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...concept. It is imperative that we all understand what antibiotic resistance is and how it develops so that we...Microbiology Pages What is antibiotic resistance? Antibiotic resistance is defined as microbial survival despite...generally organisms with multi-drug resistance are resistant to three or more different classes, or types...Learn How Antibiotic Resistance Genes Are Used in the Lab How does antibiotic resistance develop? There are...in which bacteria can become resistant to antibacterial drugs. Resistance can be intrinsic, meaning a ...means the bacterium is not naturally resistant but has become resistant somehow overtime. One fantastic example... example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics, like...

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...the resources. Instead of creating a start up budget, create a start up list.  In this post we’ll help you...most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE... much do you need to start your lab”. You can respond by saying, “The exact start up costs may vary greatly...Consulting, Inc. “How much do you need [for your lab start up]?” A hiring chair or dean will often ask a faculty...leads to poor outcomes; not enough funds to get started, wasting funds on unnecessary materials, or even... you successfully negotiate a start up award by giving you step by step instructions on creating a budget... in regards to the budget. Creating a budget We start by using your research specific aims. This is actually...

  11. Synthetic Biology - Strains

    Type
    Collection
    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back to Top Do you have suggestions...

  13. KLF Research Plasmids

    Type
    Collection
    ...efforts within the KLF community have lead to the establishment of this shared resource on Addgene. If you have...with Addgene or email [email protected] to get started. Genes KLF gene pages with information on available...

  14. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Resources Eukaryotic parasites continue to cause substantial death and disease in the world. Parasites such..., CRISPR-dCas9 gene regulation has also been established for some of these parasites. Together, these ...

  16. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless...homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require that each...

  17. Fluorescent Protein Guide

    Type
    Collection
    ... Protein Plasmids by Category Empty Backbones Understand the localization and/or function of your protein... of empty backbones. FRET Find FRET pairs and standards to study protein-protein interactions or conformational...

  18. Cancer Research Plasmids and Resources

    Type
    Collection
    ...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris...

  19. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ... Commercial DNA Ladder Penn State Ladder: Homemade Plasmid Prep Penn State Ladder: Commercial Plasmid ...undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 and pPSU2. When restriction...$200-$500 for 1000 lanes worth of ladder. The Penn State ladders are significantly cheaper. When using a ...Your Agarose Gel Other Resources Read about Gel Stains on the BitesizeBio Blog ...

  20. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene...illustrations from Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended...megabase away, or looking at the histone methylation status at a double-strand break that needs repairing. ... Pederson Phil Sharp Julie Ahringer Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard...H3.1 H3.3 HIST1H1E HIST1H2BB HIST2H2BE RBX1 STAG2 Xist Your favorite gene! SWI/SNF Genes:       ...
Showing: 61 - 80 of 1046 results