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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 852 results for: Met

Showing: 781 - 800 of 852 results

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...screens By Emily Bentley Building on their CROPseq method for optical pooled CRISPR screening, Paul Blainey...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    ...Required for Acquisition of Anoikis Resistance and Metastatic Potential in Adenoid Cystic Carcinoma, Up-Regulation...

  12. March for Science

    Type
    Blog Post
    ...scientists to be more involved in their communities (something already encouraged by “Broader Impacts” requirements...

  13. Five Popular Model Organisms

    Type
    Blog Post
    ...these cells using plasmids. Then using HOTcre, a method that uses heat induction to control the temporal...

  16. Antibody Guide

    Type
    Guide
    ...Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods Multiplex Assays ...Antibody Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows... through the indirect detection method. In the direct detection method, the signaling molecule is conjugated...Antibody Applications - Quantification Methods Quantification methods, such as western blots or ELISAs, are...output measured. This method can be performed using the indirect detection method via a conjugated secondary...sample. Controls for quantification methods Quantification methods should be run with: a positive control...included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP) and chromatin...

  17. Plan Your Experiment

    Type
    Guide
    ...delivery The first of these methods is RNA delivery of Cas and gRNA. This method involves using in vitro ...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs will... edit using DNA-based methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based...protein-based methods like a Western blot . Validation Methods There are several common ways to verify...cells. The system may be delivered using viral methods, as DNA or mRNA encoding the Cas enzyme and gRNA...Delivery Using Plasmids The most straightforward method to deliver a CRISPR system into mammalian cells... efficiently transfect. However, note that this method is most useful for cell lines that you can transfect...

  18. CRISPR Guide

    Type
    Guide
    ...toxins, cell viability, and tumor metastasis. One of the most popular methods for conducting genome-wide screens...activation through cytosine demethylation DNMT3A - repression through cytosine methylation MQ1 - repression through... to conventional methods for genomic purification, CRISPR-based purification methods are more straightforward... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes generate less off-target... editing is a “search and replace” gene editing method in which Moloney Murine Leukemia Virus Reverse ...Although lentiviral libraries are the most popular method for CRISPR screening, they are not suitable for...

  20. Cloning

    Type
    Guide
    ...including information on seven commonly used cloning methods....how do scientists recombine DNA? There are many methods that have been utilized over the years to move ...Check out our blog on choosing the right cloning method for your research project . The following guide... highlight several of the most popular cloning methods used to create recombinant DNA. Restriction Enzyme...based molecular cloning is the "classic" cloning method, and for many reasons, remains one of the most ...can be time consuming compared to other cloning methods. You can find a protocol for restriction cloning...Gateway® cloning is a recombination based cloning method. The benefit of Gateway® is that moving a piece...
Showing: 781 - 800 of 852 results