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  1. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...bandwidth to become an expert) the end result is high quality and the entire process is completed more... What types of issues do you want to know about right away? Provide adequate support. The manager must...

  2. Targeted Mutagenesis with EvolvR

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    Blog Post
    ...middle) and extends from the 3' end of the nick (right), occasionally introducing mutations (red nucleotide...EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard...

  3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...however, they are different viruses and thus use slightly different isoforms of these packaging components...research to reduce promoter interference and/or drive high expression of a transgene from an internal promoter...

  4. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...and their functions are more intuitive than you might think. So, if you’ve ever wondered what goes into...polymerase II promoter and terminator regions and are slightly different in sequence. Both LTRs do share common...

  5. Viral Vectors 101: Biosensors

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    Blog Post
    ...proteins of interest such as actinin. When tension is high vs. low in the cell the donor and acceptor of a ...on changes to the structure of actinin in low vs. high tension. These systems are powerful tools to measure...

  6. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ... and Andrew Hempstead. What do smell, taste and sight have in common, besides being one of the five senses...Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors are activated...

  9. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity... Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression ...

  12. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...microenvironments and what encircles a disease is highly prone to affect it. While tumors may be the stars...surroundings and being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated...

  13. dTAG - You're it!

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    Blog Post
    ...mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V degraders (...observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature...

  14. Summer SciComm: Preprints

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    Blog Post
    ... often post a preprint at the same time as, or slightly before, submitting the manuscript to a journal...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

  15. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ....7 kb. Co-infection rates for AAVs are generally high enough for this approach to work, although it’s ...time. Though the time delay until expression is highly dependent on the serotype and target tissue, two...

  16. Addgene: The First Twenty Years

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    Blog Post
    ...viral vectors were useful for researchers wanting high quality reagents, and we realized we could use the...Addgene’s support, could fill a community need for high-quality service, open data, and increased reproducibility...

  17. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...amino acids. The use of radioactive materials is highly regulated, requires designated work areas and come...passionate about communicating science to all audiences. Right now, she is a science instructor at The Innovation...

  20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... is biotin-conjugated, streptavidin (which has a high affinity for binding biotin), is conjugated to the...interest. The optical density (OD) - the measure of light absorbed by a solution - of these wells is plotted...
Showing: 781 - 800 of 980 results