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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 843 results for: ANG

Showing: 801 - 820 of 843 results

  3. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...red were calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation...dilutions 5–8 Note: at Addgene, samples typically range from 1 x 10 12 GC/mL to >2 x 10 13 GC/mL and we ...) ~ 1.0, E (efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will...

  4. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...drop down menu and select Scatter chart. Change the data range and select the cells where the x-axis is...Open . Choose the location of the file to open. Change the image type to 8-bit . Select Image . Select...

  5. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...the medium is acidified (i.e., the medium appears orange/yellow if using a standard phenol red pH indicator...control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4. (Link opens...

  6. CRISPR Library Amplification

    Type
    Protocol
    ... can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight. ...DNA will vary upon intended use. Typically yields range from several hundred micrograms to a milligram of...taken to perform adequate NGS based QC to ensure no change in representation compared to the pre-amplified...

  9. Weighing Reagents Protocol

    Type
    Protocol
    ...material that you are weighing by looking for a weight range on the scale. Make sure that the weight of the material... material you’re weighing out is within this range. If you need less than a gram of material, use an analytical...

  10. Protocol - Bacterial Transformation

    Type
    Protocol
    ...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific treatments...competent cells. Pro-Tips Commercial competent cells range significantly in their transformation efficiency...

  11. Immunocytochemistry

    Type
    Protocol
    ... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL of the diluted antibody... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL fluorescently-labeled...

  12. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...much as several miligrams and at concentrations ranging from 150 ng/μL to several μg/μL. The protocol below...pellet (can be done by inverting the tube at an angle over kimwipe) for 20-30 min. Resuspend pellet with...

  13. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...reverse primers are stable within this temperature range to anneal to each of the single stranded DNA template...polymerase that can withstand radical temperature changes during a typical PCR. The DNA polymerase has an...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    .... Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

  15. DNA Quantification

    Type
    Protocol
    ...measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample. Notes:...
Showing: 801 - 820 of 843 results