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... hour. Using this ratio, you can calculate the minimal amount of enzyme for your reaction. However, keep...

...for most standard cloning (where the insert is smaller than the vector) a 3 insert : 1 vector molar ratio...

...discard the tube and thaw a new working stock. The optimal mass DNA:mass PEI ratio will need to be empirically...

...the tube and vortex for 5 s to mix. Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should...

...Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient...

...higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel...

...be placed into a backbone vector of choice with minimal limitations. Background In its simplest form, PCR...

...mix. Centrifuge at 3100 x g for 8 min . Remove a small aliquot of your sample from the filter reservoir...

...incubation and plate read Tips and Troubleshooting The optimal concentration of primary antibody to use will vary...

...The health of the HEK293T cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the...