We narrowed to 810 results for: mal

1. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ... hour. Using this ratio, you can calculate the minimal amount of enzyme for your reaction. However, keep...

2. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...for most standard cloning (where the insert is smaller than the vector) a 3 insert : 1 vector molar ratio...

3. Lentivirus Production

   Type

   Protocol

   ...discard the tube and thaw a new working stock. The optimal mass DNA:mass PEI ratio will need to be empirically...

4. Transfection for Recombinant Antibodies

   Type

   Protocol

   ...the tube and vortex for 5 s to mix. Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should...

5. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ...Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient...

6. Protocol - How to Run an Agarose Gel

   Type

   Protocol

   ...higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel...

7. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...be placed into a backbone vector of choice with minimal limitations. Background In its simplest form, PCR...

8. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ...mix. Centrifuge at 3100 x g for 8 min . Remove a small aliquot of your sample from the filter reservoir...

9. Antibody Validation Using the Indirect ELISA Method

   Type

   Protocol

   ...incubation and plate read Tips and Troubleshooting The optimal concentration of primary antibody to use will vary...

10. AAV Production in HEK293 Cells

    Type

    Protocol

    ...The health of the HEK293T cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the...