We narrowed to 840 results for: Met

...from the gel via your favorite gel purification method, it is important to determine the concentration...calculate this based on DNA concentration alone. One method is to conduct 2 ligations for each plasmid you ...

...two 75 cm 2 flasks, etc. Pro-Tip This selection method results in a polyclonal cell population, meaning...screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4 (Link opens in a new window...

...reached, place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer...thermocycling. Thermal Cycling Run the following PCR parameters. Cycling Step Temperature (°C) Time (min) Ramp...

...the quality of your RNA sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer...

... protect your feet. Pro-Tip If they are able, sometimes scientists work for a long time while standing...

...often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to check this before...

...Figure 2). Both types of screen employ a selection method relevant to the phenotype being studied. Examples...

...manually call the correct base at the position. Sometimes an “N” is the result of an erroneous insertion...

...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

... some biohazardous waste, an autoclave or other method for decontaminating must be used for proper disposal...

... Introduction This protocol describes a general method for transfecting mammalian cells using linear polyethylenimine...

...your transformation procedure is working. TIP: Sometimes less is more. Although it may be counter-intuitive...

...digestion . Most restriction enzymes digest DNA asymmetrically across their recognition sequence, which results...

...incubator Pipet controller Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high ...

...; or c) loading less DNA into the well. Another method for visualizing very short DNA fragments is polyacrylamide...

...bacterial proteins and genomic DNA. Depending on the method used, the DNA concentration and purity will vary...

... Polymerase Chain Reaction (PCR) is an in vitro method that allows a single, short region of a DNA molecule...

...inverted terminal repeat sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28. (Link opens in a new window...

...right temperature, we recommend using a laser thermometer. Light the flame at the plate pouring station...

...calculate this based on DNA concentration alone. One method is to conduct 2 ligations for each plasmid you ...