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We narrowed to 971 results for: lars

Showing: 821 - 840 of 971 results

  1. Video Library

    Type
    Protocol
    ...success in numerous fields such as microbiology, molecular biology or cell biology. Learn some of the key...samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based...

  3. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... a Liquid Bacterial Culture Introduction Many molecular biology techniques require highly purified and... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...

  5. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...from academic viral vector cores. Our titers were similar to those reported by these institutions. This protocol...Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular Weight: 6208 bp x 650 dalton/bp or (g/mole)/bp...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified recombinant...

  7. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...empirically, and that the cell population be monitored regularly for transgene expression. This protocol has been...over-confluent, trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...37°C, 5% CO 2 overnight. Although cells should regularly be passaged in DMEM + 10% FBS with penicillin/...limited to the implied warranties of fitness for a particular purpose, non-infringement, typicality, safety...

  9. CRISPR Guide Update

    Type
    Blog Post
    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  10. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...

  11. The 12 Days of CRISPR

    Type
    Blog Post
    ...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th ...

  13. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...community of creative scientists delivered some spectacular photos once again this year! All winners receive...

  14. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... 101: Una herramienta de edición  Addgene has a large international audience, including working with distributors...

  17. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...
Showing: 821 - 840 of 971 results