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We narrowed to 1,035 results for: LAS

Showing: 841 - 860 of 1035 results

  1. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...Recombinant Antibodies You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection... intended to support reproducibility in science. Last Update: June 14, 2023 Workflow Timeline Day 1: Run...Rocking platform Fume hood Metal spatula Razor blade Plastic tray Gel imaging system ImageJ or similar software...in the microcentrifuge. Remove the gel from the plastic wrapper and rinse with deionized water. Gently ...Section 2: Staining the Gel Place the gel in a plastic tray with 100 mL of deionized water. Rinse gel ...

  2. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...1) and biosafety level 2 (BSL-2). The BSL-1 classification is for labs working with low-risk microbes ...not be possible depending on the type of sleeve. Lastly, don’t touch your face! Close-toed shoes and long... be used as needed. For BSL-2 work always wear glasses/goggles in addition to the BSL-1 requirements. ...here has been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene...

  3. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...good idea to place the gel on a glass plate if available. Unlike the plastic tray, this will not significantly...Gel You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis DNA Ligation Background...for your eyes!), remove it from any gel tray as plastic will block much of the UV and with a clean, sterile...

  4. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection You may...Receptor Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene Description AR plasmids AR, RP11...receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA)... Nuclear Receptor plasmid collection for the community. If you have created plasmids that you would like...ESRRB Plasmids ESRRB, DFNB35, ERR2, ERRb, ESRL2, NR3B2 estrogen-related receptor beta ESRRG Plasmids ESRRG...group A, member 1 PGR Plasmids PGR, NR3C3, PR progesterone receptor PPARA Plasmids PPARA, SC22CB-5E3.5,...receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha RORA Plasmids RORA, ROR1, ROR2...

  5. Fluorescence Titering Assay

    Type
    Protocol
    ...media Day 4–5: Count fluorescent cells Equipment Class II, Type A2 Biological Safety Cabinet 0.5–10 µL ...obtaining accurate titers. Check the cells for mycoplasma (Link opens in a new window) regularly Do not...mCherry expression using fluorescence microscopy. Last reviewed on: September 9, 2023...

  6. Immunocytochemistry

    Type
    Protocol
    ...Immunocytochemistry You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western... intended to support reproducibility in science. Last Update: January 20, 2022 Workflow Timeline Day 1... the protein, such as cells transfected with a plasmid to express the protein of interest, and a negative...

  7. Colony Formation Titering Assay

    Type
    Protocol
    ...Stain cells and count resistant colonies Equipment Class II, Type A2 Biological Safety Cabinet 0.5–10 µL ...obtaining accurate titers. Check the cells for mycoplasma (Link opens in a new window) regularly Do not...single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

  8. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...PCR) You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background...detailed instructions and check out our protocol on plasmid cloning by PCR to learn how to design primers for...

  9. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Protein A or Protein G You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection...following your institution’s hazardous waste procedures. Last Update: February 7, 2022 Protocol Video Watch this...antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator...

  10. Lentivirus ddPCR Titration

    Type
    Protocol
    ...compliance with your institution’s biosafety regulations. Last Update: July 7, 2023 Workflow Timeline Day 1: Seed...harvest cells Day 4+: ddPCR and analysis Equipment Class II, Type A2 Biological Safety Cabinet Aspirating...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL...

  11. DNA Quantification

    Type
    Protocol
    ...Culture Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information...

  12. Ligation Independent Cloning

    Type
    Protocol
    ... does not provide enough stability to hold the plasmid together through the transformation/replication...reaction is shown. Mix the components (add the T4 last) and incubate at room temperature for 30 minutes...

  13. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Indirect ELISA Method You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibodies 101: ...plotted against the micrograms of antigen loaded. Last reviewed on: October 8, 2024...

  14. AAV ddPCR Titration

    Type
    Protocol
    ... institution’s biosafety regulations. Equipment Class II, Type A2 Biological Safety Cabinet, Labconco ...on AAV titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

  15. Plasmid Collections

    Type
    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 159,176 plasmids . We've...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael... Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

  16. Chemogenetics Plasmids

    Type
    Collection
    ... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...: Chemogenetics vs. Optogenetics Chemogenetics plasmids encode genetically engineered receptors that are...processes. Addgene’s collection contains two main classes of chemogenetic receptors: Designer Receptors Exclusively...which are inhibitory. Another recently developed class of tools are Luminopsins (LMOs), or luminescent ...

  17. MAPK Plasmids

    Type
    Collection
    ... Plasmid Collections MAPK Plasmids MAPK Plasmids You may also like... Human...has assembled a MAPK plasmid collection for community. If you have created plasmids containing MAPK and...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...protein kinase (MAPK) plasmids. Plasmid...addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene Alternate Names MAPK1...

  18. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of medium-sized...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

  19. Lentivirus Plasmids

    Type
    Collection
    ... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table...packaging plasmids that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation...be used with 3rd generation transfer plasmids along with a plasmid encoding Rev Trono 12260 psPAX2 2nd ...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...

  20. Retrovirus Plasmids

    Type
    Collection
    ... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...
Showing: 841 - 860 of 1035 results