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We narrowed to 895 results for: plasmid

Showing: 841 - 860 of 895 results

  1. Zinc Finger Consortium Reagents

    Type
    Collection
    ...like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe Lab plasmids The Zinc Finger Consortium... Plasmid Collections Zinc Finger Consortium Zinc Finger ...Technologies TALEN Engineering Reagents CRISPR Plasmids...

  2. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...download from each plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism...collaborations between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE...and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several...the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the..."active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources...opens in a new window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and...

  3. Antibodies

    Type
    Collection
    ...Looking for Antibody Plasmids? Browse our collection of affinity reagent plasmids - including antibodies...affinity reagents are expressed and purified from plasmids in our repository. Antibodies...collection of affinity reagents that are expressed from plasmids. These antibodies undergo application-specific...antibodies, nanobodies, scFvs, and more! Find Plasmids...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...that have used these constructs experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...genetic variants and disease associations. Plasmids This plasmid collection contains codon-optimized ORF .... Browse the SLC plasmid collection. Depositor... Depositor Collections Resolute RESOLUTE Plasmid Collection You may...Brain Initiative Collection NeuroMab Collection Plasmids Pooled Libraries Feedback Solute carrier proteins... range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR...

  6. Rett Syndrome

    Type
    Collection
    ...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

  7. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...these types of studies Addgene has a collection of plasmids suitable for creating individual fluorescently...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial... to Top FRET Reference Standards The following plasmids have well-characterized FRET efficiency values...

  8. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...microbial contamination. Purity When possible, all plasmids used for viral production are propagated in the...experiment using our virus protocols . Browse our plasmid collection of popular lentiviral vectors ....

  9. Serotype Testing AAV

    Type
    Collection
    ...AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct ...AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...a sample of the purified DNA from the original plasmid that was used to make the virus. For more details...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  10. CRISPR References and Information

    Type
    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning... (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators... proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21...

  11. Cre-lox system

    Type
    Collection
    ... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...viral preparations of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression...loxP plasmids, reporter genes indicate which cells have undergone Cre recombination. These plasmids can...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Cre-lox plasmids for your experiments. Plasmid...more thorough introduction, check out Addgene’s Plasmids 101: Cre-lox blog post. Based on these Cre-lox...

  12. mTOR Pathway

    Type
    Collection
    ...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...

  13. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ...and phenotype and shares their plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication...Browse plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects...genomic variants on genome function and phenotypes. Plasmids The collection will grow as the IGVF community...

  14. Lentiviral Prep Service

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    Collection
    ...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,... Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and... protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described...However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell ...

  16. p53 Pathway

    Type
    Collection
    ...function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...

  17. All Antibodies

    Type
    Collection
    ...provide important details including the protein and plasmid sequence, the antigen sequence, and cross-species...develop and refine these application lists. The plasmids we use to produce antibodies are available as ...as part of Addgene’s larger Antibody Plasmid Collection , which also includes other types of affinity reagents...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or email...

  18. Plan Your Experiment

    Type
    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...the expression time (transient or stable). For plasmid and viral-based approaches, the species and expression...

  19. Ras Pathway

    Type
    Collection
    ...human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...mTOR Pathway p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small... - Gene List Click on a name to find available plasmids for the gene. For components with multiple subunits...

  20. Antibody Production

    Type
    Collection
    ... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light chains. Antibodies...control and the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody...the Sanger method, and aligned with the parental plasmid sequence to confirm the antibody’s identity. For...
Showing: 841 - 860 of 895 results