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We narrowed to 885 results for: TRI

Showing: 861 - 880 of 885 results

  1. Ligation Independent Cloning

    Type
    Protocol
    ...Empty vectors for LIC typically employ type II restriction enzymes (e.g. BsaI), which cut at a specified...overhangs with a single enzyme, and remove the restriction sites from the final product (no "cloning scars...sequence and sacB counter-selection marker (see our restriction digest protocol ). After the digest is complete...corresponding to the first base removed by the restriction digest to create the free 5' end of the cut vector...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ...presence of the antibiotic). Luria broth (LB) is a nutrient-rich media commonly used to culture bacteria in... are unable to digest the agar but can gather nutrition from the LB within. The addition of an antibiotic...manufacturer to make sure this is true in your case. Retrieve your molten agar mix from the autoclave. Pro-Tip...technique. Swirl the agar bottle to ensure even distribution of the antibiotic throughout the agar. Open ...

  3. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...DNA from an Agarose Gel You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis...technique for molecular cloning, such as PCR - or restriction enzyme -based cloning. Protocol: Gel Purification... the excised band, lay it down on the UV box and trim the top, bottom and sides with the razor blade. ...

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost any two fragments regardless...least the correctly assembled antibiotic gene. This trick can also enable replacement of "inverse PCR" reactions...and screen for the correct plasmid product by restriction digest . Sequence the important regions of your...

  5. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Electrophoresis uses an electrical field to move the negatively charged DNA through an agarose gel matrix toward a positive...Agarose Gel Electrophoresis You may also like... Restriction Digest of Plasmid DNA Purifying DNA from an Agarose...

  6. Protocol - How to Design Primers

    Type
    Protocol
    ...complementary regions Note: If you will be including a restriction site at the 5’ end of your primer, note that ...the enzyme to cleave efficiently (e.g. GCGGCG-restriction site-your sequence). Protocol Video Watch the...

  7. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...aware that some gloves may contain latex. Consider nitrile gloves as an alternative. For extra precautions...guidance is also adhered to. None of the authors, contributors, administrators, or anyone else associated with...

  8. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ..., however, more expensive. With a 6 cm diameter petri plate containing solidified LB-agar, pipette 150...used for this purpose, provided that they fit your petri plate. Incubate the plate at room temperature for...

  9. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... visible in the room and on the door. BSL-2 centrifugation steps require an aerosol-tight lid and rotors...guidance is also adhered to. None of the authors, contributors, administrators, or anyone else associated with...

  10. Lentivirus Production

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 Microcentrifuge tubes, Neptune 3745.X 10 cm tissue culture ...storage tube and store at 4 °C between harvest. Centrifuge the viral supernatant at 2100 rcf for 5 minutes...

  11. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background...rate of the DNA polymerase. Deoxynucleoside Triphosphates (dNTPs): Are the building blocks added one at...

  12. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...DNase 10X DNase buffer Nuclease-free water Microcentrifuge tubes 96-well optical plate Pipette tips General...least 5 times. Seal plate with transparent film. Centrifuge at 3,000 rpm for 2 min to bring the sample to...

  13. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...pipette reagent reservoirs Pipette controller Microcentrifuge Microplate shaker ELISA plate washer (optional...clear) microplate seal, Thermo Scientific 5701 Microcentrifuge tubes, Neptune 3745.X Pipette tips, 1000 µL...

  14. DNA Quantification

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information ...

  17. Weighing Reagents Protocol

    Type
    Protocol
    ...0.02 g to resuspend in 1 mL), you may place a microcentrifuge tube on the balance and then tare it. Then ...

  18. Water Bath Protocol

    Type
    Protocol
    ...temperature, catalyze chemical reactions such as restriction digests needed in molecular biology, and thaw...

  20. Fluorescence Titering Assay

    Type
    Protocol
    ...preps, if prep was not previously filtered) Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture...
Showing: 861 - 880 of 885 results