We narrowed to 1,052 results for: FER
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TypeBlog Post...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...
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Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post... investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An... of the scientific research infrastructure. References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S,... -
Hot Plasmids - January 2023
TypeBlog Post...Facility Isotype IgG2a Differences: Distinct variable domains that recognize different epitopes on GFP Monoclonal... -
25 Work From Home Tips for Scientists
TypeBlog Post...time for exercise. Many studios and gyms are now offering online classes and participating in these is a... Jennifer Tsang... -
5 Great Apps for Lab Life
TypeBlog Post...restriction enzymes since the instructions use NEB buffers. Nonetheless, if you are setting up a double digest... provides a list of directions including which buffers to use and that indicates if a sequential digest... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...various scientific or engineering applications. References Refactoring and optimization of light-switchable... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene... of how miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post... proteins whose excitation and emission spectra differ sufficiently for them to be imaged in distinct ...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there... -
Plasmids 101: Inducible Promoters
TypeBlog Post...and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only ...Experimental System Because transcription machinery differs between cell types or organisms, promoters are ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post... and precisely identifies plasmid features that differ by only a few nucleotides, such as the fluorescent...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024. References and Resources Further reading Harnessing homologous... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage, codon usage, and expression...Visit our COVID-19 Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped... -
Viral Vectors 101: Types of viruses
TypeBlog Post...high and your immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer... -
Hot Plasmids - May 2022
TypeBlog Post...peptide. By combining three different localization peptides with four different fluorescent proteins, and... -
Antibodies 101: ChIP
TypeBlog Post... the cutting. While sonication is usually the preferred method, enzymatic digestion can be appropriate...protocols when embarking on your ChIP journey! References and Resources More resources on Addgene’s blog... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post... web of opportunities that the classroom cannot offer. Furthermore, companies across most industries tend...helps you find internships and scholarships in different subject areas! 2. Resume and cover letter... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...culture). This dilution step represents the major difference between PACE and PANCE: for PANCE the phage progeny...much more efficiently and in more contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...Listen to the C. elegans RNAi podcast segment RNA interference (RNAi) is extensively used in C. elegans research...endogenous RNAi pathways. These mutations however can interfere with other genetic pathways making it difficult... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...al. applied TRIM-away to nine proteins in ten ddifferent cell types, including difficult-to-manipulate...that TRIM21 overexpression did not affect cell proliferation. These cells also did not contain changes in...