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...editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to me: the PEDAR strategy...

...Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts...

...annual #Deckthelab contest. The bar was set very high from last year’s impressive entries, but our community...

...with you.  Top 5 science comics:   1)   “Piled Higher and Deeper” by Jorge Cham   2)   “Boxplot” by Maki...

...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5...colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers...dilutions, and single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

...in 8,900 institutions across 111 countries. Just eight days into 2024, we've already shipped 2,078 items...

...journey. And if you have additional resources that might be helpful, please reach out to me by email.  About...

...to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving past... P1000 pipette shows the volume adjustment ring right below the plunger. This ring changes the pipette...P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume P2 0.2 to 2 µL P10 1 to...

...organized by graduate student researchers. That’s right, #ActualLivingScientists, eager to communicate their...

...topic page Read Joachim's Series on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing...

...purified AAV. Option #3 Puncture the QuickSeal tube slightly below the 60–40% interface with an 18 ga needle... (1999): 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers... viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-PAGE gel of...

...and career goals, or to answer any questions you might have about Addgene. The page is divided into three...

...the Expression and Insert Species filters on the right-hand side of the search page to narrow your keyword...

...plasmids at Addgene, along with other tools like high resolution Ras sensors and crosslinked peptides ...

... University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as a lecturer...

... about Crystal’s exciting career path, to gain insight into industry research, and to get advice on how...

...finally warming up in Boston and that means summer is right around the corner. Here at Addgene, we’re always...

...winner will be determined by popular vote. That’s right - you get to choose the new name! Additionally, ...

...of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes, hang...

...fluorescent protein family that give off color in ambient light. Expressing these chromoproteins in E. coli would...