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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 939 results for: TIM

Showing: 921 - 939 of 939 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... of Solution II and invert the tube carefully 5 times to mix the contents. The contents will become clear...Solution III to each tube. Mix by inverting several times. A white precipitate will be formed which contains... 0.1 mM EDTA). Pro-Tip DNA resuspension can take time, it is a good idea to let it sit for several hours...

  3. Protocol - How to Streak a Plate

    Type
    Protocol
    ...it through a flame, just be sure to allow enough time for the loop to cool before touching it to the bacteria...often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to check this before...

  4. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...will want to use long-wavelength UV for as short a time as possible to get the bands cut out. Once you have...the gel at a lower voltage for a longer period of time; b) using a wider gel comb; or c) loading less DNA...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ...weigh out 100 g three times, placing the sucrose in your container after each time. Place the weighing ...

  6. Ligation Independent Cloning

    Type
    Protocol
    ...Independent Cloning (LIC) obviates the need for the time-consuming ligation step of traditional cloning methods...sterile water (instead of TE buffer) to ensure optimal salt concentrations in subsequent reactions. Step...

  7. Centrifugation

    Type
    Protocol
    ...place, and the control panel where you can set the time and speed needed for your experiment. Procedure ...Adjust the centrifuge settings according to speed and time (and temperature, if applicable) listed in your ...

  8. Fluorescence Titering Assay

    Type
    Protocol
    ... institution’s biosafety regulations. Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells ...with multiple integration events leading to underestimation of the true titer. Calculate the transduction...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...can work fine in an assembly if you want to save time. Working on ice, combine segments in the Gibson ...rate when assembling more than five fragments at a time. Resources and References New England Biolabs (NEB...

  10. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece...both function in the same buffer, as this will save time later. In our example, we will use EcoRI and NotI...

  11. DNA Quantification

    Type
    Protocol
    ... the absorbance of a liquid you can accurately estimate the concentration of a substance in that liquid...

  16. Video Library

    Type
    Protocol
    ...offer some tips and tricks to reduce transformation time and increase efficiency Bacterial Transformation...

  18. Pouring LB Agar Plates

    Type
    Protocol
    ...antibiotic throughout the agar. Open one plate at a time next to the flame and begin pouring. Measure your...
Showing: 921 - 939 of 939 results