We narrowed to 958 results for: Tes

1. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

   ...envelope plasmid you use as the choice of envelope dictates the tropism of the virus.  VSV-G is very common...

2. Twenty Years of Sharing: Addgene's Viral Vector Service

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   Blog Post

   ...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...

3. Plasmids 101: Plasmid Incompatibility

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   Blog Post

   ...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...

4. How to Write a Scientific Review Article

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   Blog Post

   ...was most productive in short bursts (even twenty minutes was enough to make some progress). For most researchers...

5. Viral Vectors 101: Preparing Pooled Libraries

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   ...amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed...

6. 5 Reasons to Use Reddit for Science Communication

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   Blog Post

   ...too many to describe here, but I have over 2,000 notes which Evernote even allows me to access on my phone...

7. CRISPR Activation: A Practical Guide

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   Blog Post

   ...you want to activate, you should also be ready to test three or four guides directed towards that gene ...

8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

   ...vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting...

9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang D, Baltes NJ, Paul JW III, Tang X, Zheng X, Voytas DF, Hsieh...

10. Plasmids 101: Repressible Promoters

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    Blog Post

    ... the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling it to bind...

11. Typing CRISPR Systems

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    Blog Post

    ... clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is already on...

12. How to Deposit Your Plasmids with Addgene

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    Blog Post

    ...descriptions first before entering the data. This step associates an Addgene ID with your plasmids, and adding ...

13. Protocol - How to Design Primers

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    Protocol

    ...also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process...

14. Protocol - How to Create a Bacterial Glycerol Stock

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    Protocol

    ...2 mL screw top tube or cryovial and gently mix. Notes: Make the 50% glycerol solution by diluting 100%...

15. Protocol - How to Streak a Plate

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    ... Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...

16. Fluorescence Titering Assay

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    Protocol

    ...lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one ...

17. Lab Safety for Biosafety Levels One and Two

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    Protocol

    ... in a final concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste...

18. Isolating a Monoclonal Cell Population by Limiting Dilution

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    Protocol

    ...PES filter or centrifugation at >500 x g for 5 minutes. Do not use the medium if the cells are overly ...