... and forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants...

...in target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its...

...on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what do you do? — Stephani Page, ...

...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target...

... disciplinary communities gather at ComSciCon's local and specialized franchise workshops throughout the...

...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing!  A recent...

...m always catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss ...

...of an open-access recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The...

... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...

...effects of inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM...

...from the Neuroscience Information Framework (NIF) called the Resource Identification Initiative. During ...

...With our protocol, it is feasible to produce genetically modified cells in less than a month's time. Addgene...

...EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA May contain reporter gene (e.g. ...needs to match the target locus, but it is also critical to ensure that the gRNA target sequence does NOT...target with no homology elsewhere in the genome. Realistically, a given gRNA target sequence will have partial...additional sites throughout the genome. These sites are called off-targets and should be examined during gRNA ...

...dosage in E. coli using DIAL strains." Journal of biological engineering 5.1 (2011): 10. PubMed PMID: 21787416...

... the desired level of antibodies is reached - typically within 2-4 months. After the animal has the desired...

...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...

...too long in the incubator. The small colonies are called satellite colonies and they form when all the antibiotic...

... targeting the Fc region of an antibody, the so-called ‘constant’ region which is, quite usefully, both...

... types of proteins. They started with another biological workhorse, Cre recombinase, which induces recombination...