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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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  1. Pipetting Protocol

    Type
    Protocol
    ...containers Reagents Liquid for pipetting Background Information Anatomy of a Pipette This front view photo of...the Volume on the Pipette Although each pipette performs the same function, the numbers are read differently...

  2. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and...Watch the protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic material...of experiments being performed. Your biosafety office can provide more information on your institution'...they are different viruses and require different isoforms of these packaging components. Therefore, gamma-retroviruses...ccdB-resistant strain is necessary. For more information on cloning and working with plasmids, visit Addgene... the gamma-retroviral genome and the genetic information encoded by that genome. Antibiotic resistance...expanded into a cell line. You can find more information in Addgene’s stable cell line protocol . Addgene... Addgene’s Biosafety Resource Guide for more information and resources on viral safety. Resources and ...

  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration....Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence Titration...

  5. Chemogenetics Guide

    Type
    Guide
    ...PSAM is coupled with an ion pore domain (IPD) to form ligand-gated ion channels (LGICs) that can either...

  6. DNA Quantification

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA Background Information During several different stages of molecular ...

  7. Protocol - How to Design Primers

    Type
    Protocol
    ... capabilities. Taking into consideration the information above, primers should generally have the following...

  8. Protocol - How to Streak a Plate

    Type
    Protocol
    ...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...

  10. General Transfection

    Type
    Protocol
    ... (am): Observe fluorescence, harvest cells, or perform your experiment Equipment Class II, Type A2 Biological...

  11. Gibson Assembly Protocol

    Type
    Protocol
    ...You can purchase master mix or make your own. Transform bacteria with the DNA and screen for the correct...

  12. Lentiviral Vector Guide

    Type
    Guide
    ...infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic material...of experiments being performed. Your biosafety office can provide more information on your institution'...they are different viruses and require different isoforms of these packaging components. Therefore, lentivirus...ccdB-resistant strain is necessary. For more information on cloning and working with plasmids, visit Addgene...will have the lentiviral genome and the genetic information encoded by that genome. Antibiotic resistance...GFP and later expanded into a cell line. More information on generating cell lines with lentiviral vectors... Addgene’s Biosafety Resource Guide for more information (including tropism and viral genome percentage...

  15. AAV ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration....

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ...serotypes are harvested at 96–120 h. Optional: perform a media exchange at ~18 h post transfection. Changing...

  17. Guide to Using Pooled Libraries

    Type
    Guide
    ...your library from Addgene, check the library information to see if it should be amplified before you conduct...in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS) on a control sample...library Apply selection; most cells will survive Perform NGS on the surviving cells Compare NGS results ... Company Resources Science Exchange Scientist (formally Assay Depot) Resources Find additional resources...amplification protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse...

  18. Educational Resources

    Type
    Guide
    ...Selection What is a Plasmid? Genetic Code Strain Information Molecular Cloning Techniques Protocols Find written...virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning Creating Bacterial Glycerol...

  19. Addgene's eBook Collection

    Type
    Guide
    ...find tips for choosing a fluorescent protein; information on FRET, biosensors, and monitoring protein localization... | First Edition About The guide includes information on the many career options in science, advice...

  20. Promoters

    Type
    Guide
    ...molecule binds to the repressor, it changes its conformation, preventing its binding to the operator and ...
Showing: 941 - 960 of 960 results