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Showing: 941 - 960 of 965 results

  1. CRISPR Guide

    Type
    Guide
    ...ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions...effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3 for more details...with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation...dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble...Cas9 undergoes a second conformational change upon target binding. In this formation, its nuclease domains...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into an active,...

  2. Molecular Cloning Techniques

    Type
    Guide
    ...repetitive sequences and DNA that ends in sequences that form complex secondary structures. Read more in our SLIC... with four nicks that bacteria repair after transformation. LIC does not require site-specific recombination... is rendered unnecessary. The product may be transformed directly into E. coli , where the normal replication... recombination abilities of yeast. By simply transforming into yeast two (or more) fragments of dsDNA ...than 10 kb. Another advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA... seamless manner. To accomplish this, you can transform into yeast the fragments of DNA to be fused along...

  3. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... Gel Electrophoresis DNA Ligation Background Information Gel purification allows you to isolate and purify...step with the now gel purified DNA. Find more information about DNA quantification here . Tips and FAQ ...

  5. Pipetting Protocol

    Type
    Protocol
    ...containers Reagents Liquid for pipetting Background Information Anatomy of a Pipette This front view photo of...the Volume on the Pipette Although each pipette performs the same function, the numbers are read differently...

  6. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and...Watch the protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well...

  7. Modular Cloning Guide

    Type
    Guide
    ...modules can be assembled into a Level 2 vector, forming a functional genetic circuit. Level 2 vectors are...E. coli to maintain the final plasmid after transformation. Figure 1: Overview of the MoClo Toolkit components...multigene constructs. MoClo Plant Parts III: Transformation & Genome Engineering Kit Plant Expression, ...plasmids with multi-gene constructs for plant transformation. GoldenBraid 2.0 Kit Plant Expression, CRISPR...Daniel Voytas 76 plasmids for assembling plant transformation vectors for overexpression, gene editing, functional...Expression, Mammalian Expression Stephan Gruber Platform for rapid assembly of expression vectors for multi-subunit..., Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome editing in the yeast...

  8. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration....Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence Titration...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic material...of experiments being performed. Your biosafety office can provide more information on your institution'...they are different viruses and require different isoforms of these packaging components. Therefore, gamma-retroviruses...ccdB-resistant strain is necessary. For more information on cloning and working with plasmids, visit Addgene... the gamma-retroviral genome and the genetic information encoded by that genome. Antibiotic resistance...expanded into a cell line. You can find more information in Addgene’s stable cell line protocol . Addgene... Addgene’s Biosafety Resource Guide for more information and resources on viral safety. Resources and ...

  10. Chemogenetics Guide

    Type
    Guide
    ...PSAM is coupled with an ion pore domain (IPD) to form ligand-gated ion channels (LGICs) that can either...

  11. DNA Quantification

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA Background Information During several different stages of molecular ...

  12. Protocol - How to Design Primers

    Type
    Protocol
    ... capabilities. Taking into consideration the information above, primers should generally have the following...

  13. Protocol - How to Streak a Plate

    Type
    Protocol
    ...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...

  15. Gibson Assembly Protocol

    Type
    Protocol
    ...You can purchase master mix or make your own. Transform bacteria with the DNA and screen for the correct...

  16. General Transfection

    Type
    Protocol
    ... (am): Observe fluorescence, harvest cells, or perform your experiment Equipment Class II, Type A2 Biological...

  19. AAV ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration....

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...serotypes are harvested at 96–120 h. Optional: perform a media exchange at ~18 h post transfection. Changing...
Showing: 941 - 960 of 965 results