...In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have...

...is important to know that the student presenting right before me had been assigned a structure paper with...

...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about...

...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....tissue culture treated dish, VWR, 29442-042 DMEM, high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated... Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium...increases by a factor of ~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown...

...western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ...

...education. If you suspect that science communication might be for you, don’t wait. Start communicating now....

...for a lay readership, and working with middle and high school students. One of these organizations was ...

...species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ...

...your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing...

...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...

... job appealed to us. However, since the lab was highly focused on protecting intellectual property, it...

...spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength... absorbance, you need to know the wavelength of light that your substance maximally absorbs. In the case...

...Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated...

...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever...

...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared...

...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target...

...with one or more of these parameters can lead to a high number of false negatives in Y2H screens. Testing...

...bacteria deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number...