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We narrowed to 994 results for: Des

Showing: 961 - 980 of 994 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes how to...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

  2. Science Guides

    Type
    Guide
    ...Collection of guides that describe how popular scientific techniques work... Science Guides Addgene has created... created the following guides to help you start learning about various scientific concepts related to ...to our plasmid collection. Find links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics... sets. Read More Viral Vectors Find educational guides to learn more about the different types of viruses...

  3. DNA Quantification

    Type
    Protocol
    ...samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

  4. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...and with a clean, sterile razor blade, slice the desired DNA fragment from the gel. Notes: To protect the...down on the UV box and trim the top, bottom and sides with the razor blade. This is especially important...

  5. Lentivirus Production

    Type
    Protocol
    ...Lentivirus Production You may also like... Viral Vector Guides Mol Bio Protocols Introduction This protocol can...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

  6. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Ultracentrifugation You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction...preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules. The...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ...-agar 1 L Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which...next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the first plate...

  8. Kit Free RNA Extraction

    Type
    Protocol
    ... Biology Reference Introduction This protocol describes how to extract RNA from tissues or cells without...precipitation, and resuspension. This protocol provides two options: using Solution D (see reagent section...

  9. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Guide Virus Blog Posts Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

  12. Fluorescence Titering Assay

    Type
    Protocol
    ...for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...

  14. Colony Formation Titering Assay

    Type
    Protocol
    ...for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...

  18. Addgene's eBook Collection

    Type
    Guide
    ...First Edition, trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close About... tips for planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide...November 2018 | First Edition About The guide includes information on the many career options in science...

  19. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Retroviral Vector Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S...PMID: 24501411 Gilroy, K. L., Terry, A., Naseer, A., De Ridder, J., Allahyar, A., Wang, W., Carpenter, E.... Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral...been widely used in the research community for decades. Wildtype gamma-retroviruses have been engineered...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...lentiviral packaging methods . The three plasmids described above (envelope, packaging, and transfer) are ...into the HEK293T packaging cell line. This system provides the greatest flexibility to pseudotype gamma-retrovirus...

  20. CRISPR Guide

    Type
    Guide
    ...M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries, R., Brouns, S. J., & Van Der...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including...Han, Y., Ogrodowski, P., Crippa, A., Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014)...., Boix, C., Perrouault, L., Tesson, L., Geny, S., De Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli...application (see: gRNA Design Software ). In addition to an optimized gRNA design, engineered Cas9s can... the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit...
Showing: 961 - 980 of 994 results