We narrowed to 1,039 results for: LAS

...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. Convert...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...decision-making, and language abilities. The disease can be classified as early-onset, where symptoms appear between...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and...

...Grade DNA (cgDNA) for over 200 plasmids in our collection. The plasmids available in this format include...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as...individual plasmid page to find current pricing information Standard Quality Control Plasmid verified by...next generation sequencing Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ...grade DNA preps of this plasmid available, but helps to inform us of which plasmids would be most helpful... our most popular plasmids! DNA... DNA Service: Cloning Grade DNA Service Details Plasmids Available FAQ Addgene...

...reporter) pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-...activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654 and 159655...constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids 175573 and ...expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert a second guide... #110821 ) VPR ( Addgene #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...

... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available... a new window) Last reviewed on: 18 Jun 2025 Do you have suggestions for other plasmids that should be...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid... Plasmid Collections...Collections ARF Superfamily Plasmids for ARF Superfamily Research You may also like... RAS Clone Collections Richard...

...., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., Tsai, J. C., Weissman, J. S., Dueber, ...different classification of nucleotide base (purine-to-pyrimidine and vice versa) The first classes of base...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

...Synthetic Biology Plasmids MoClo Guide Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Blog: Golden Gate...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 ...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology... (Link opens in a new window) . How to Use The plasmids in the Open Enzyme collection contain genetic ...are recommended). The selection marker for all plasmids in this collection is ampicillin. Find more information...Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514...

...Fluorescent Protein Collection Luciferase Plasmid Collection Promega Plasmid Collection Botman-Teusink Yeast Fluorescent...Lambert Content last reviewed: 25 September 2025 Do you have suggestions for other plasmids that should be...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization...Emission (nm) Brightness pKa Maturation Structure Plasmids AcGFP1 475 505 28 Prone to dimerization AcGFP1...

... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This new version of ZiFiT...REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional pages...Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ...TALENs, and assembly methods for REAL, REAL-Fast and FLASH Genome...method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput...

...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...type, species, and more. Plasmids Browse the table below to see all of the plasmids that are currently available...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity...Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts ...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...that have used these constructs experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...

...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic... Plasmid Collections Genetic Code Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the... ncAAs. This plasmid often carries multiple tRNA copies for efficiency. A target plasmid containing the...NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that contain aminoacyl-tRNA synthetases... of these plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase...Bacterial TAG Wei Niu Return to top Target Plasmids Browse plasmids that contain genes with modified codons...

...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...bacterial strains, including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Synthetic Biology Synthetic Biology...Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...

...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype is...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure..., while others exist as monomers. Each antibody class therefore has a valency, or number of binding sites...

...Proteins: Plasmids and Resources Addgene's repository contains thousands of fluorescent protein plasmids. Use...proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty...Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell Science Plasmid Collection New England BioLabs... BioLabs Cell-Imaging Plasmid Collection Promega Plasmid Collection Botman-Teusink Yeast Fluorescent Protein...and to find the plasmids that are available from Addgene's depositing scientists. Plasmid... Plasmid Collections Fluorescent...Resources at Addgene Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit, and control molecular...

...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes...2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert... pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 ...

...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE...(Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Control Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination... tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages before being...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Synthetic Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table...

... more. Empty Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return...Return to top Cyan Plasmid Description Industry Return to top Green Plasmid Description Industry Return...Return to top mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return...Return to top Orange Plasmid Description Industry Return to top Red Plasmid Description Industry Return... fluorescent proteins. Plasmid Description Industry Return to top Content last reviewed: 25 September ... Plasmid Collections Fluorescent Proteins Michael Davidson...proteins with your gene of interest. Most of these plasmids are available to industry scientists as well as...

...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene...applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter ...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven serotypes...important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...