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We narrowed to 1,035 results for: LAS

Showing: 961 - 980 of 1035 results

  1. Synthetic Biology - Overview

    Type
    Collection
    ...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...bacterial strains, including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Synthetic Biology Synthetic Biology...Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...

  2. AAV Packaged on Request

    Type
    Collection
    ...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene...applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter ...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...lets scientists choose from over 3,000 transfer plasmids to be packaged in their choice of five serotypes...2.5 mL, or 5 mL). Currently, we can only package plasmids in our collection in the serotypes listed here...

  3. Lentiviral Prep Service

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    Collection
    ...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes...2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,...

  4. CRISPR References and Information

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    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE...(Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning...

  5. Viral Production

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    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Control Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination... tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages before being...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...

  6. Distribution to Industry

    Type
    Collection
    ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...to Industry Featured Collections Search Plasmids Browse Plasmids Browse Antibodies Ordering and MTA Information...see all of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts ... ready-to-use recombinant antibodies made from plasmids in our collection! These monoclonal antibodies...

  7. Michael Davidson Fluorescent Protein Collection

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    Collection
    ... Backbones Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description...Industry Blues Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description...Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Fluorescent Proteins Michael Davidson...

  8. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Synthetic Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table...

  9. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive... Other Plasmids Bas Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression Resources...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs ...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter...

  10. Genomic Deletions in Mammalian Cell Lines

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    Collection
    ...usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and... protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described...However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell ...

  11. Genetic Code Expansion

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    Collection
    ...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced in the media...

  12. Ras Pathway

    Type
    Collection
    ...human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...mTOR Pathway p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small...Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact Ras is ubiquitously expressed... - Gene List Click on a name to find available plasmids for the gene. For components with multiple subunits...v-akt murine thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho...

  13. Trimmer Lab NeuroMab Collection

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    Collection
    ...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References... Antibody Plasmid Collection NeuroMab Plasmid Collection...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...Mouse IgG2a 114542 Anti-LRP4 (cytoplasmic) [N295B/66R] LRP4 (cytoplasmic) Mouse Mouse IgG2a 114544 Anti-GABA...Mouse IgG2a 177555 Anti-ADAM22 (cytoplasmic) [N46/30R] ADAM22 (cytoplasmic) Mouse Mouse IgG2a 177556 Anti-Rufy3...

  14. Fluorescent Protein Guide

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    Collection
    ...Fluorescent Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP...Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide ...applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing scientists... of fluorescent proteins (FPs) and to find the plasmids that are available from Addgene's depositing scientists... scientists. Browse Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization...genetically encoded fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control...with either actuators or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically...

  15. Fluorescent Protein Guide: In Vivo Imaging

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    Collection
    ... empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging...Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Plasmids for in vivo imaging using far red, near-infrared, and photoactivatable fluorescent proteins.... Plasmid Collections Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In Vivo Imaging...imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670 642/...

  16. TALEN Expression Vectors

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    Collection
    ...and FLASH You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids This...repeat arrays assembled using the REAL, REAL-Fast or FLASH methods TALengineering...This page lists plasmid vectors into which DNA fragments encoding TAL effector repeat arrays (assembled...assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details...details about the REAL, REAL-Fast, and FLASH assembly methods can be found on the TALengineering website...with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit . Vectors...domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI...

  17. Brain Initiative Collection

    Type
    Collection
    ...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...research. Browse the BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...dissemination to the research community. Browse plasmids , viral preps , and antibodies created with support...distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as high-quality...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage reporter... pZac2.1-GfaABC1D-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000...IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR...

  18. Ginkgo Bioworks COVID-19 Collection

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    Collection
    ... entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed ...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...Browse plasmids encoding SARS-CoV-2 viral proteins. Depositor...Ginkgo Bioworks COVID-19 Plasmid Collection (Link opens in a new window) Researchers developing novel ...Addgene has partnered with Ginkgo to make these plasmids available to scientists in academic and industrial...) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional ...Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems for a summary of the...

  19. Allen Institute for Brain Science AAV Enhancer Collection

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    Collection
    ...Sevigny JP, Shapovalova N, Shulga L, Sigler AR, Siverts LA, Somasundaram S, Stewart K, Szelenyi E, Tieu M, Trader...Bendrick JL, Kim TK, Zhou T, Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader C, Balaram...Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids which use genomic regulatory... Tools Atlas (Link opens in a new window) . Allen Institute for Brain Science Enhancer Plasmids The Allen...Science Enhancer Collection contains best-in-class AAV plasmids to target a variety of cell populations in...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...details, visit the Allen Institute's Genetic Tools Atlas (Link opens in a new window) . Figure 1: Selection...

  20. Mammalian RNAi Tools

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    Collection
    ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources ...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral... occur via transfection or viral delivery of a plasmid. shRNAs are often delivered using viral vectors...with some retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that...inducible (Tet) expression are available. To find plasmids containing other RNAi components, like argonaute...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...
Showing: 961 - 980 of 1035 results