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We narrowed to 84 results for: pooled crispr library plasmids dna

Showing: 41 - 50 of 84 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read ... These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-...Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out our updated CRISPR-Cas ...CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been...

  2. CRISPR Pooled gRNA Libraries

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    Collection
    ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ...virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single...the steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments...about each library can be found on the individual library page. Find Pooled Libraries of Interest...custom filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode...

  3. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos to specific DNA loci John...Addgene Blog Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts ...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ... control over CRISPR/Cas9 mediated genome modification and gene expression. The plasmids associated with...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...whereas the nonsequestered pool goes on to initiate translation. These TRICK plasmids provide valuable tools...

  4. CRISPR Activation: A Practical Guide

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    Blog Post
    ...(2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods... function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences...using oligo library synthesis. Prior to Cas9 activators, similar tools were made using other DNA binding ...group has develop several popular CRISPR-based tools for altering DNA sequence or regulating gene expression..., Wang X (2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome... More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on...Catch Up on Your CRISPR Background with Our CRISPR Guide Pages ...

  5. CRISPR Plasmids - Parasites

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    Collection
    ... Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...

  6. CRISPR Plasmids and Resources

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    Collection
    ...used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout, activation...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed.... C. elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for ... Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for... parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use...

  7. CRISPR Guide

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    Collection
    ...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...For more detail on using CRISPR libraries for screens, see our pooled library guide and our blog post ...

  8. CRISPR References and Information

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    Collection
    ... Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...Description Plasmids in protocol Download protocol Addgene CRISPR pooled library amplification CRISPR pooled...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled Libraries Pooled Library Name Type PI...Suppressor Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase...Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent...Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits You may also like… AAV Enhancer Collection...Chemogenetics Plasmid Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids... cleave all four DNA strands at once. They can mediate the integration of a large DNA payload containing...same DNA strand in opposite directions, recombination results in an inversion and the region of DNA between...

  10. COVID-19 Resources

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    Collection
    ...Cas12a plasmids Addgene's full CRISPR guide and summary of CRISPR pooled libraries . FlipGFP-based activity...useful for detection assays and more. Pooled Libraries : Pooled libraries of spike protein mutations. Research...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...SARS-CoV-2 plasmids are curated on a separate Ginkgo Bioworks Plasmid Collection page. SARS-CoV-2 Pooled Libraries...to ask them to deposit the plasmid with us. Return to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...
Showing: 41 - 50 of 84 results