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Showing: 121 - 150 of 782 results

  1. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...tweet from the conference is shown above. The blog post I mentioned, John Doench’s How to Design your gRNA... repair pathways. An emphasis on cellular repair post-CRISPR cleavage When I first looked at the conference...forget this in the future. Here’s a list of blog posts to help you tackle the challenges of DNA repair ...across multiple systems. Read our introductory blog post, then check out this RNP protocol on Protocols.io...higher the efficiency of HDR repair. Check out this post to see if an engineered Cas9 variant might work ...

  2. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...information that I decided to write a few blog posts. This first post will be based on recycling! With the popularity...Sign up for blog posts and updates from Labconscious. If you’re inspired by this post then I challenge...Find lab tips for your research Read another blog post about conservation in the lab Resources on Addgene.org...the box at any location where the United States Postal Service picks up mail. NEB actually started this...box, flip the flaps of the box so the pre-paid postage stamp is on the outside of the container, and drop...the box at any location where the United States Postal Service picks up mail. Recycle plastic packaging...

  3. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...In this post in the Careers in Science Communication blog series, you’ll learn about Caitlin Runne-Janczy... her job at eScience Labs is like. Find all the posts in this series here. Caitlin Runne-Janczy Writing...teaching experiences definitely weighed in Caitlin’s post-grad plans, but in the end, writing won out. “I ...Channel of the blog so you don’t miss the future posts in this series!  Additional Resources on the Addgene...school And just for good measure, here’s a few other posts about developing transferable skills while in grad...” In the short term though, she did a one year postdoc in the Biochemistry Department at Iowa, but kept...to be a much better writer.” At the end of her postdoc, Caitlin had a clearer idea of her career goals...

  4. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ... Look at master's-level job postings  Look at master’s-level job postings on a job search website like...see yourself enjoying?  To find master’s-level postings, try adding “master’s” to your favorite search...technical writer,” among others. Don’t discount job postings looking for PhD applicants since many will also...decision, but hopefully the questions in this blog post can help you start to figure out if a master’s is...

  5. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...time meeting with and talking to graduate students, post-docs, and PIs. I won’t pretend that that there aren...process of writing, editing, and publishing blog posts runs its course more quickly than writing scientific...warned me, the swift gratification of publishing posts can be addicting. Even more satisfying, because ... enjoyed: travel. I’m actually finishing up this post on a plane back to Boston from Kentucky where I ... PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about Management...

  6. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...E-Book format at the end of this post. If you have been following the posts in this Mentoring for Scientists...This is the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series... the extensive resource list available from this post. Use an activity to start a discussion. The Forced...which includes the entire series of mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer...graduate student can mentor an undergraduate, a postdoc can mentor a graduate student – you get the idea...

  7. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  8. Science Communication for Everyone

    Type
    Blog Post
    ...This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. ...e-magazine this year which would feature articles, posts, and sci-art that would ultimately give a visual...A contribution can either be an article, a short post, a video, or a piece of sci-art.  What are your ...Addgene blog Find all of our science communication posts Sign up to receive our science career guide Learn...science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive... Sandangi! Dr. Chinmaya Sadangi is currently a postdoc at the University of Toronto and founder of The...

  10. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...In this post of the Careers in Science Communication blog series, you’ll hear from Susan Keown, a staff...skill set wasn’t matching up with the local job postings. “There were jobs that I didn’t seem to have the...Twitter feeds and dealing with social messages and postings,” says Susan. There are also team meetings and...often easier to accept unpaid work as a student. For post-grad school individuals, Susan recommends starting...the blog so you don’t miss out on future career posts! Additional Resources on the Addgene Blog Volunteer...Communication Check out Other Science Communication Posts Resources on Addgene.org Explore careers at Addgene...sitting in my office one day, which I shared with a postdoc, and I’m like, “Oh my god, what can I do?! This...

  11. The Twelve Days of CRISPR

    Type
    Blog Post
    ...keeps changing, we’ve been updating many of these posts over the last several months. Now the #12DaysOfCRISPR...Twitter where we shared one of these updated blog posts each day. If you missed it, here’s the recap! The...series. Be sure to subscribe to the CRISPR blog posts so you don’t miss a thing! ...

  12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...This post was contributed by guest blogger, Gary McDowell, executive director of Future of Research. ...increasing from $23,660 to $47,476 per year. This post discusses how a nationwide injunction against the..., while 69% of all postdocs were expected to receive salary raises, 6% of postdocs were to have either...tracked, 3% of postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...on the morale of postdocs, and indeed we have been contacted by a number of postdocs who are now planning...salaries, the only postdocs we knew for certain to still be getting their raise were: postdocs at institutions...carry over into other aspects of postdocs’ lives. We know that many postdocs already made plans and budgets...

  13. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some are...researchers from the Boehm and Beroukhim labs to write a post on their paper uncovering ancestral bias in CRISPR...targeting to animal models. It was one of our favorite posts from 2022! Wow! Our depositors do some really incredible...

  14. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...This post is part of our ongoing Plasmids 101 series. Plasmids 101 will provide you with an overview ...more, you can find the rest of the Plasmids 101 posts here. Now that we have covered antibiotic selection...strategies, check out our screening overview blog post.) *Note: Two Popular Vectors for Blue White Screening...phenotype. Resources on the Addgene Blog Read Our Post on Common Lab E. coli Strains Learn to Use Yeast...Two Hybrid Systems Browse All of Our Plasmids 101 Posts Resources on the Addgene Website Choose the Best...

  15. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  17. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens...used effectively in a published article. This blog post will serve as a guide to use our validated gRNA ...this shared resource can continue to grow. This post was written jointly by Brook Pyhtila and Nicole ...CRISPR Software Matchmaker Browse Alll of Our CRISPR Posts Additional Resources on Addgene.org Browse gRNA...

  18. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...first post in a three-part series. We’ll first discuss how to get started on fly work in this post. The...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....The second post will detail a major tool used by Drosophila geneticists (the Gal4/UAS system), and the ...the third post will describe how you can make your own mutant flies. Find Drosophila Resources at Addgene...gene knock down and gene expression. In the next post, you’ll learn the fundamentals about how many of...

  19. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...selection in this blog post, but don’t worry, we’ll cover screens in a future post. Positive selection...naturally occurring genes  At this point in the blog post, you may have realized that positive and negative...on the Addgene blog Read our Plasmids 101 blog posts Learn more about Lambda Red recombineering Learn...

  20. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...scientists up to on Twitter? Prior to writing this post, my interest in Twitter was fleeting. I’ve had an...8 tweetable facts. Note: The images used in this post were created using data from or modified from Ke...Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains more often ... use social media as a scientist? Check out this post. Resources on the Addgene Blog 6 Tools & Tips:...

  21. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest bloggers. Check...Check out this blog post for more information. Additional resources on the Addgene blog Download the...the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  22. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...antibiotic resistance marker). In a previous blog post, we covered how to use positive and negative selection...are blue. Check out our blue-white screening blog post for more information.   Figure 1: Result of... process, or visit our restriction analysis blog post. Colony PCR Colony PCR can detect the presence...details and learn more about colony PCR in this blog post.   Figure 2: The steps of colony PCR.   ...either terminus. Want to know more? Visit this blog post for tips for analyzing and troubleshooting Sanger...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...

  23. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...million plasmids shared milestone in 2018, we wrote a post on the time and cost required to make a plasmid.... not!), we think it’s about time we updated this post. How much time does it take to make a plasmid?...precise calculations for any of the numbers in this post is a mathematical story well beyond my ability to...help you choose the right cloning method Find blog posts about scientific sharing here Download the Plasmids...save yourself hours by chatting quickly with the postdoc who’s really good at primer design – but first,...

  24. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance...of plants and animals. Unlike bacteria, yeast can post-translationally modify proteins yet they still share...Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed how the regulation of bacterial...

  25. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...biology blog posts Find tips for Arabidopsis transformation Read our Plasmids 101 blog posts Resources ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with...transient technique that uses a virus and the plant's post-transcriptional machinery to silence genes of interest...MoClo system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo...and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene silencing method." International...

  26. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...connecting. You can see what they post and occasionally comment on their posts as appropriate, possibly giving...companies and organizations to see their news and job postings. 4) Use LinkedIn to pay it forward The best way...profiles. 7) Keep it professional at all times Do not post or comment on anything that is not relevant to business...for using social media as a scientists Read blog posts about networking ...will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile” instead of “...

  27. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts Resources...seem endless - and overwhelming. In the first blog post in this series, I’ll highlight terminology you should...mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing and maintaining...

  28. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...lower the probability of off-target editing. In this post, we’ll summarize how IDT (Integrated DNA Technologies...nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, ...donors (if possible) The text and images in this post were adapted from an IDT webinar: Optimized methods...Mollie Schubert and Shuqi Yan for reviewing this blog post. Additional Resources on the Addgene Blog Learn...

  29. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...tools not listed in today’s post? Share them in the comments below. This post was written in April 2020 ...virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology...functional information, such as expression patterns, post-translational modifications and polymorphisms Codon...

  30. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...In this post of the Careers in Science Communication blog series, you’ll hear more about Caitlin Runne-Janczy...how Caitlin got into scicomm and landed her first post-grad school job, head over to part I of the interview... proposals, a few PhD theses, and occasionally posting something science-related on Facebook. “I think...Channel of the blog so you don’t miss the future posts in this series! Additional Resources on the Addgene...
Showing: 121 - 150 of 782 results