We narrowed to 253 results for: Mac

1. Clear and Accessible Writing

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   ...through increasingly dense layers until the macromolecule density is equal to the buoyant force of the...

2. GCE4All: Making Genetic Code Expansion Accessible

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   ...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...

3. Viral Vectors 101: Viruses as Biological Tools

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   ...associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows...

4. Sharing is Caring: But How to Distribute Open Hardware?

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   ...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation...

5. GPCRs: How Do They Work and How Do We Study Them?

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   ...some GPCRs further to respond to specific, pharmacologically-inert, small molecules. These new receptors...

6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...podcast segment As the primary cause for both the stomach flu and food-borne illnesses, noroviruses can infect...

7. Plasmids 101: Inducible Promoters

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   ...promoter. Experimental System Because transcription machinery differs between cell types or organisms, promoters...

8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...provides greater spatiotemporal resolution than pharmacological or genetic manipulation of the signaling pathway...

9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell...

10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...efficient gene deletion in B cells, T cells, macrophages, and dendritic cells, with no deleterious effects...

11. CRISPR 101: Non-Homologous End Joining

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    ..., repressing RecA and overexpressing the NHEJ machinery improved NHEJ accuracy in M. smegmatis (Yan et...

12. Viral Vectors 101: An Introduction to AAV

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    ...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...

13. All in a Twist: dsRNA

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    ...foreign dsRNA activates the innate immune response machinery of the host. This pathway has been clinically...

14. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...10.1073/pnas.0700293104 Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (...

15. RNA Extraction Without A Kit

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    ...0005-2744(74)90240-x Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically...

16. Human Germline Editing Using CRISPR

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    ...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...

17. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...provide greater spatiotemporal resolution than pharmacological or genetic approaches, but only a few such ...

18. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...samples. RNA is inherently less stable than most macromolecules used in scientific research such as DNA or ...

19. Working with Nuclear Receptors

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    ...physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity purification...

20. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ... sgRNA to temporarily block the transcription machinery of a target gene or DNA that codes for ncRNA. ...

21. CRISPR in the Clinic

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    ...for these diseases was sponsored by Vertex Pharmaceuticals and CRISPR Therapeutics. Data shared from these...

22. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...Lavine CL, Zuo T, Lin J, Zuiani A, Feldman J, MacDonald EA, Hauser BM, Griffths A, Seaman MS, Schmidt ...

23. How to Prepare for an Industry Interview

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    ...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ...

24. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...can express siRNAs to direct the cellular RNAi machinery to silence target genes. This empty MuLV vector...

25. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ... through physical blocking of the translation machinery. In the Sánchez lab protocol, dsRNAs are delivered...

26. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...PMCID: 3308701. Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet the neighbors...

27. Tips for Technical Support Calls

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    ... delicate balance of knowledge, patience, and diplomacy, and that balancing act is made much easier if...

28. Adapting Toehold Switches to Detect Zika Virus

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    ...Assistant professor in the Leslie Dan Faculty of Pharmacy at the University of Toronto. His research is ...

29. Plasmids 101: Terminators and PolyA signals

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    ... newly synthesized RNA from the transcription machinery. Terminators are found downstream of the gene ...

30. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ...replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no proteins). The chemistry...

31. The Materials Science of Optogenetics Experiments

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    ...Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics. Cell 162.3 (2015): 662-674...

32. Common Injection Routes in Mice

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    ...experimental therapies or pain relief, straight to the stomach. This technique involves administering medication...

33. Magnetic Control of Proteins: More than a Dream

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    ...., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical...

34. Antibodies 101: Flow Compensation

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    ... Inputting compensation values differs between machines or the program you’re using, but usually you can...

35. Plasmids 101: Degron Tags

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    ... tag, and the endogenous cellular degradation machinery to function. The dTAG is the smallest tag reviewed...

36. Transferable Skills: Negotiation

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    ...that the brand new grad student keeps using the machine? Let’s walk through a more specific example....

37. Google Forums Round Up: First Impressions of NgAgo

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    ...which might be rapidly degraded by the cellular machinery, leaving a small amount of active NgAgo/ssDNA ...

38. Light Sheet Fluorescence Microscopy

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    ...215. Pubmed. W. Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten...

39. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ... compared with Google Docs and wikis - by Rory Macneil on Bitesize Bio Data Freedom: The Expansion of ...

40. An Introduction to Adenovirus

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    ... Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl, H. C. J. (2007). Adenoviral...