...red light rapidly deactivates the system. Version 1 of this far-red/red light-responsive system is spread... also be used to engineer new pathways with sophisticated gene expression controls. Most cell types do...light; these two systems can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive...

...know that most cephalopods die after they lay their 1 clutch of eggs? Propagation is thus quite difficult...organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2 ...communities using them grow. So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both...

...Figure 1). Clustered events are called a population.                             Figure 1: While..., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS ...

...Strong mammalian promoter from human elongation factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter...promoter for small RNA expression UAS Specific Drosophila promoter containing Gal4 binding sites Bacterial...

...manipulation, there are two main decisions to make: 1. Which modification? and 2. Which viral vector should...years, with historical ties to psychology and philosophy (the mind-body problem). Because of the availability...function directly such as receptor subunits or neurotrophic factors. Another option uses optogentics to ...

...education, government, and civilization in general (1). Science and politics? Science is an apolitical process...consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that the ...Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values and their ...

...mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent expression... from Drosophila Actin 5c gene Constitutive  Commonly used in expression systems for Drosophila. Polyhedrin...containing Gal4 binding sites, commonly used in Drosophila Specific Requires the presence of Gal4 gene to...

...downstream experiments. You only successfully generate 1 null clone and proceed with it. In this case, the ...there are 10 putative off-target locations you can 1) determine if editing at those sites would confound...for mismatches within the gRNA sequence. Graphic courtesy of Maya Kostman. The most commonly...

...plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells ... the type II CRISPR3 module of Streptococcus thermophilus (the popular Streptococcus pyogenes system does...

...plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system. Genome wide mouse...not possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which were ...

...social media? No problem - see our recap below! Day 1: gRNA design tools: How to Design your gRNA for CRISPR... category. Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on...

...started:  Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one thing...involved in education advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your...Radio, WIRED, Scientific American or National Geographic. Similarly, media fellowships from the British...

... inhibitory synapses in the brain.  Figure 1:Schematic diagram of different FingR constructs and...over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively in... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...

...in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while...INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade...identified the Cas9 orthologue from Streptococcus thermophilus, Cas9sth1 as a robust and highly active enzyme... al., 2014). These could be genes, specific polymorphisms, including antibiotic resistance genes. This...

...to western blotting  In a western, proteins are: (1) separated by size, (2) transferred to a membrane,...  (3) detected using antibodies.   Figure 1: Overview of the Western blot process. The sample,...sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein...

...could be kept frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box...sampling trip to Yellowstone National Park (YNP). Extremophiles - phylogenetically diverse microbes living in...

...in online patient/caregiver communities  Table 1: Examples of defining your audience for science communication...ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed… really...

...relations, advocacy, and writing legislation (see Figure 1 for career map). The beautiful part of science policy...about science policy as a profession. Figure 1: Science policy can lead you to many paths! For a ...approval for a new product or a well-endowed philanthropy who can shift the funding landscape to focus...

... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ...approach that exploits the bacteriophage integrase phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory...2018): 967-968. PubMed PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic...

... difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a...DNA of interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free...

...variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

... cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne ...Networking connections can last years This is a graphic snapshot of my LinkedIn network from a few years...

...heterodimerizes with CIBN upon blue light exposure (see Fig 1*). When the group targeted the enhancer region of ...further develop dCas9 for synthetic looping, the Phillips-Cremins lab developed the light-activated-dynamic-looping...

...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...coagulation factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...

...individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which will be ...meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD...

...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

...and CasPEDIA have got you covered!     Fig. 1 – CasPEDIA Cas ID display information for a given ...to relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas...

...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...found bound to IgE receptors on the surface of basophils and mast cells, two types of white blood cells...

... UK team members before they log off for the day. 1:45 PM--Write final report. Work on a final report ...what the day to day looks like. Check out the infographic for an outline of my day or find the text version...

...prove the technology. This transition takes around 1 – 2 years. The product is the measure of success...Business - Posted on Nature.com, contributed by Julia Phillips and Fiona Beckman Acknowledgments Thank you ...

... trafficking of these biosensors.   Figure 1: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay... calcium signaling related fluorescence during morphine administration and withdrawal to explore how these...

...see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...

...first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple catalogue...follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al. "Genome editing in the human... not. It is an offline tool, but comes with a graphical user interface. Best strategy for labs: Define...

...significant progress in clinical trials.     Figure 1: Types of delivery and editing strategies for CRISPR...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

...viruses - 2 cas systems regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University...invasion.  His lab is studying P. furiosus, a hyperthermophile that exists in sea vents at the boiling point...

... – a mechanical device that could be used to bend 1/8th inch steel wire into a backbone structure of a...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...

...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...relies on heat resistant DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase...

...editing machinery such as CRISPR-Cas. Figure 1: Schematic of targeted gene knock-out (left) and targeted... WM, Curiel DT (2019) Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9. Journal...

...genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse genome...shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma than the standard...