We narrowed to 438 results for: ERG

1. With an Eye Towards the Future, We Look Back at the March for Science

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   ...Rensselaer Polytechnic Institute. He also studies energy markets in his spare time. You can follow him on...are not present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be...

2. Rett Syndrome

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   ...1147. (Link opens in a new window) PMID: 17289941 Hagberg et al. 1983. A progressive syndrome of autism, ...

3. CRISPR 101: Multiplex Expression of gRNAs

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   ...hypopigmentation, thus marking cells that have undergone genomic modification. In this system, Cas9 must...plasmids from this paper at Addgene. 8. Xie, Kabin, Minkenberg, Bastian, & Yinong Yang. “Boosting CRISPR/Cas9...

4. CRISPR 101: Cytosine Transversion Editors

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   ...generations of optimization before the field begins to converge on a set of standard tools. Additionally, aspiring...Base Editing. Advanced Science (Weinheim, Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002...

5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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   ...editor proteins, but found that the mutations that emerged depended strongly on the target edit (Doman et .... E., Holden, K., Ma, H., Xie, J., Gao, G., … Gootenberg, J. S. (2023). Drag-and-drop genome insertion...

6. Antibody Validation for Flow Cytometry

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   ...Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, D. L., Rodriguez, H., Hiltke...

7. Retrograde AAV viral preps

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   ...standard viral production , these viral vectors undergo quality control, including AAV titering by SYBR...

8. Caltech Systemic Capsids

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   ...standard viral production , these viral vectors undergo quality control, including AAV titering by probe-based...

9. Using AAV for Neuronal Tracing

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   ...in only specific cell types, for example only dopaminergic neurons, neuronal tracing has the added benefit...798–810. PubMed PMID: 92475. Taymans, J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C....

10. Tetracycline Inducible Expression

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    ...Tet-On plasmid for differentiating iPSCs into glutamatergic neurons via human NGN2 expression. See more ...

11. Troubleshooting and Optimizing a Western Blot

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    ...compartment, you may want to try an SDS-containing detergent like RIPA. You can try a few different lysing ...background noise is high, you can consider adding a detergent like Tween-20 to your washes — although this can...

12. Fluorescent Protein Guide: Subcellular Localization

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    ...genetic material. The mitochondria produces the energy cells required for chemical reactions. Labeling...

13. 22 Hot Plasmid Technologies from 2014

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    ...mitochondrial degradation in vitro and in vivo. Merged green and red channel fluorescence images of C2C12...effectively cuts the target sequence, the plasmid undergoes HR or SSA to reconstitute functional GFP, and ...

14. CRISPR Plasmids - Empty gRNA Vectors

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    ...sgRNA-scaffold 47912 Worm AflII none S. pyogenes Sternberg pJW1311 61254 Worm none, fusion PCR none S. pyogenes...

15. Antibody Guide

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    ...against parasites and is responsible for driving allergic reactions such as anaphylactic shock Monomer with...

16. 27 Hot Plasmids from 2016

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    ... et al. Science. 2013. PubMed PMID: 24136966 Nyerges, et al. Proc Natl Acad Sci USA. 2016. PubMed PMID...Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue proteins with SpyTag/SpyCatcher and SnoopTag/SnoopCatcher...

17. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

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    ...Toolbox  Hot Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new...

18. The 12 Days of CRISPR

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    ...day of CRISPR, Addgene gave to me: RADARS from Gootenberg, Chen, and Abudayyeh🎵On the 9th day of CRISPR...

19. Lucky Thirteen: Guest Contributors of 2023

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    ...Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew...

20. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

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    ...dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing plasmid Didier...

21. 8 Great Depositor Research Stories

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    ...Cas7-11 and PASTE: Omar Abudayyeh and Jonathan Gootenberg worked with us to share their new CRISPR tools...

22. DIY DNA Ladders from Penn State University

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    ... recently deposited with Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed...

23. New Viral Vectors - March 2024

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    ... a biosensor that detects both serotonin and serotonergic drugs. Check out these new vectors, and many...

24. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

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    ...shown to be at high risk by these screens can then undergo more routine screening allowing for early cervical...

25. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

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    ...countries often find themselves on the front lines of emerging global challenges. However, these same researchers...

26. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

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    ...involved in supervising many successful iGEM undergraduate projects. Special thanks to our sound engineer...

27. MassBioEd Career Exploration Day at Addgene

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    ...Senior Scientist Joel McDade’s presentation on the emerging CRISPR genome editing technology was a rousing...

28. What's New in CRISPR - May 2019

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    ...per gene and includes ~10,000 gRNAs targeting intergenic regions. The libraries also include 2,400 gRNAs...

29. Making Career Development an Integral Part of the Science Training System

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    ...business degree. From the very start of their undergraduate experiences, they were provided with ample resources...

30. Inntags: Innovative Protein Epitope Tagging

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    ...protein. Since this process takes a lot of time and energy, Martí Aldea and collaborators have created a set...

31. Science Communication for Everyone

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    ...Live Q&A session on Instagram every Sunday. Undergraduate and graduate students are more than welcome ...

32. Fluorescent Proteins 101: History of Fluorescent Proteins

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    ...with new colors Around the same time, researcher Sergey Lukyanov realized that, although they are not bioluminescent...

33. Developing Transferable Skills During Science Training

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    ...professional experience are prized skills. Supervise undergrads or less experienced grads Manage a project involving...

34. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

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    ...mouse, human or plants,” explained Jan Lohmann of Heidelberg University. “What is different is the plasmids...

35. Bringing Sustainable Practices to the Lab: Innovations

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    ...environmental impact in terms of manufacturing, energy, and water use. ACT labels are obtained by independent...

36. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    ...understanding of how cancers become resistant, an emerging theme is that they do so by activating signaling...

37. Management for Scientists: Seeking Feedback

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    ...another, and their conversations and gestures are energetic.  This can take practice and training. This is...

38. From our Table to Yours: An Inside Look at Lunch at Addgene

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    ...will forgo their regular To-Do lists (other than emergency items) and focus on a single project. These projects...

39. Six Spooky Science Stories and Halloween at Addgene

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    ...insect host to dive into water. There, the worm emerges to find a mate, leaving the insect dead or dying...

40. SciComm with the Experts at Science in the News Part 2

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    ... recently covered inlcude CRISPR, mosquitoes, allergies, & more! We briefly answer the question, will ...

41. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

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    ... Waxmonsky. References McDade J, Waxmonsky NC. Emerging CRISPR Technologies Available Through Resource...

42. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

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    ...great Mentor is often one that devotes time and energy to thinking about how to be a great Mentor.    ...

43. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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    .... Blugene, our trusted friend and mascot, has undergone a make-over as well. While in the course of redesigning...

44. Working 9-5: an Addgene Co-op

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    ...the Addgene blog A Guide to Getting Started in Undergrad Research Tips for Writing a Good Cover Letter ...

45. 2023 and 2024: Reflections and Looking Ahead

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    ...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity...

46. New Norepinephrine Indicators: nLightG and nLightR

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    ... nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted...

47. Scientific Sharing in the Time of COVID-19: Databases and Resources

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    ...scientific sharing is important for creating research synergies and accelerating the translational impact of research...

48. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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    ...The panel was moderated by a non-scientist from Bloomberg news. Given the topic it might have been better...

49. 9 Tips to Achieve Success in Academia

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    ...get caught up in the hype. 3. “Passion gives you energy.” What is your passion? Even if no one else is ...

50. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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    ...pivotal role in spontaneous swimming. References Arrenberg, Aristides B., Filippo Del Bene, and Herwig Baier...