... check out our Biosensors Plasmid Collection . To find optogenetics plasmids available through Addgene...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids for your next experiment. For a ...domain (iLID plasmid), while its natural binding partner SspB is included in a separate plasmid; Blue light...dimerization tags (TULIPs), plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb ...photoreceptor for light-controlled gene expression. These plasmids rely on the engineered two-component ...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...

...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains ...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description Origin...biology basics and plasmid components. Plasmids 101: Origin of Replication Plasmids 101: The Promoter ...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...

...Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector...72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from ...pCDH-EF1-Nluc 73024 Express Nluc from the EF1 promoter pCDH-EF1-copGFP 73030 Expresses copGFP from the EF1 promoter...pCDH-EF1S-Nluc 73033 Expresses Nluc from a truncated EF1 promoter pCDH-EF1s-copGFP 73034 Expressed copGFP from a truncated...pAdx-CMV-copGFP 73346 Expresses copGFP from the CMV promoter pAdx-CMV-tdTomato 73347 Expresses tdTomato...pAdx-EF1-copGFP 73353 Expresses copGFP from the EF1 promoter pAdx-EF1-tdTomato 73354 Expresses tdTomato...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under from...

...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly...circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped...reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...

...zebrafish Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous...CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish... Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) for use in mammalian ...endogenous human genes Guide RNA expression plasmids for endogenous zebrafish genes...RNA-Guided Nuclease (RGN) Plasmids Genome... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...

...direct virus infection specifically into cells that express the TVA viral receptor. Thus, by pseudotyping the...virus with EnvA, only neuronal cells engineered to express TVA will be infected. Schematic depicting steps...to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped RAVB is delivered... via G-protein receptors, which are naturally expressed at presynaptic nerve terminals. (4) Neurons projecting...G-protein coated RABV. These projection neurons do not express G-protein, and thus do not give rise to G-protein...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies...

... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional...

...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein...a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be helpful...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may...

... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. ...TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and FLASH ...Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL Assembly...REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to...FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast and FLASH TALENs for Endogenous...Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome editing...

... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available... have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid...

...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...

... Plasmid Collections Pleiades Promoter Plasmids Pleiades Promoter Project ...using the links to view individual plasmid webpages. These plasmids are described in Portales-Casamar ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective...selective expression in the brain. Plasmid...human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below. Additional information ...regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 ... pEMS1301 cre/EGFP/NLS N/A pEMS1308 EGFP/cre N/A pEMS1302 EGFP/cre/NLS N/A pEMS1306 EGFP/NLS N/A pEMS1307...

...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...