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We narrowed to 261 results for: gnal

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CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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Blog Post

...circuits with many guide RNAs connected because the signal degrades at each layer. If we could fix these things...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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Blog Post

...JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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Blog Post

...primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can bind to a ...
Fluorescent Proteins 101: Introduction to FRET

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Blog Post

...small molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular...
Plasmids for Endogenous Gene Tagging in Human Cells

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Blog Post

... function and result in a ubiquitous background signal that makes it hard to visualize the precise location...
Hot Plasmids February 2024

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Blog Post

... to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing...
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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Blog Post

...outspoken in their opposition to unions, potentially signaling a rough road ahead for students interested in ...
Plasmids 101: Protein Expression

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Blog Post

...University and is particularly interested in cell signaling and communication. She loves being able to help...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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Blog Post

...Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification RANbodies: Reporter Nanobody...
Hot Plasmids and Viral Preps - May 2021

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Blog Post

...enriched in the pre-synaptic terminal providing better signal to noise for imaging calcium dynamics in fine structures...
The AAVantages of AAV in CRISPR Screens

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Blog Post

...delivery vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy, 9(1). https:...
Magnetic Control of Proteins: More than a Dream

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Blog Post

...expressed and purified. Although a ~1% change in signal might not make a very powerful tool, it was promising...
Antibodies 101: Flow Compensation

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Blog Post

...telling your cytometer to adjust the fluorescent signals by a specific value between the two channels. It...
Cloning Mammalian Cells with the Agarose Method

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Blog Post

... Baltimore. She is particularly interested in signaling peptide maturation in neural and endocrine cells...
Plasmids 101: Multicistronic Vectors

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Blog Post

....0018556. Epub 2011 Apr 29.) PubMed. 5. Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs ...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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Blog Post

...typically invisible information into actionable signals. Let's say I wanted to detect every primary metabolite...
Simplify Cloning with in vivo Assembly

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Blog Post

...Molecular Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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Blog Post

...Marvin E., et al. "A protein-tagging system for signal amplification in gene expression and fluorescence...
Tips for Screening with Yeast Two Hybrid Systems

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Blog Post

...negatives: Interactions leading to detectable reporter signals depend on a number of factors, including protein...
Plasmids 101: Protein tags

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Blog Post

...of a bacterially derived in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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Blog Post

...inducible translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004...
Your Lentiviral Plasmid FAQs Answered

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Blog Post

...HIV components: LTRs, PPT, RRE, and psi packaging signal. Viral components typically total <1.5kb, which...
Analyzing CRISPR Editing Results with ICE from Synthego

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Blog Post

.... It shows, base-by-base, the average amount of signal that disagrees with the reference sequence derived...
Deciphering the Mysteries of Behavior with Viral Vectors

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Blog Post

...stress response circuit by integrating neuronal signalling and triggering release of the stress hormone ...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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Blog Post

...presence of liver metastasis by producing a detectable signal in urine. The bottom line with plasmid addiction...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

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Blog Post

...3-5 nucleotide PAM sequence serves as a binding signal for Cas9 and this sequence is a strict requirement...
CRISPR 101: Targeting RNA with Cas13a (C2c2)

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Blog Post

... activation of the fluorophore. The fluorescent signal can be used as an indicator to determine whether...
Hot Plasmids - November 2023

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Blog Post

...with a phorbol ester activator gave fluorescent signal in the ticker tape from cFos::GFP-iPAK4 within ...
CRISPR Activation: A Practical Guide

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Blog Post

...JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence...
Tips for CRISPR Gene Editing in Mice

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Blog Post

...target is inserted, disrupting the EGFP fluorescent signal, for details on this plasmid, see Mashiko et al...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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Blog Post

...color as the fused XFP can be used to amplify the signal. Imaging endogenous fluorescence in live animals...
Five Popular Model Organisms, Part 2

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Blog Post

...Gardiner, 2011). As many biological processes and signaling pathways that regulate regeneration and wound ...
Antibodies 101: Introduction to Immunofluorescence

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Blog Post

...molecules can bind to a single primary, resulting in signal amplification. Another reason is cost effectiveness...
Filming Science Videos in the Age of Social Distancing

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Blog Post

...inexpensive HDMI transmitter that could transmit a signal between a wall. There was a SLIGHT delay, but I...
Using Phosphoserine to Study Protein Phosphorylation

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Blog Post

...particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You...
Evolution of Lab Techniques

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Blog Post

...result can be quantified by the strength of the signal in comparison to a control sample. Southern blotting...
How to Design Your gRNA for CRISPR Genome Editing

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Blog Post

... that off-target effects did not overwhelm true signal in these assays (Dempster et al., 2019). Again,...
Antibodies 101: The Basics of Western Blotting

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Blog Post

... the primary antibody and amplify the detection signal. Analyzing the western blot Once visible, it’...
Plasmids 101: Repressible Promoters

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Blog Post

... working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline...
Technique: Probe Phage Genomes for Host Binding Proteins

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Blog Post

...lead to high background that obscures the desired signal. We have successfully corrected this by incubating...
Recombinase-based State Machines Enable Order-dependent Logic in vivo

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Blog Post

...information regarding which regulatory proteins, signaling molecules, mutations, and environmental conditions...
Generating Mouse Models Using CRISPR/Cas9

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Blog Post

... it gets exciting – the cell sends out an “SOS” signal and cellular repair mechanisms rush in to repair...
PiggyBac-ing Through the Genome Editing Field

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Blog Post

...expressed has threshold effects, such as with signaling molecules or transcription factors, or when the...
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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Blog Post

... volume. The consequence is that you can detect signal in thick specimens. See the video below for an ...
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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Blog Post

...absence of photoactivation resulting in an increased signal to noise ratio. PA-FPs with higher contrast ratios...
Special Delivery: Fluorophore Targeting for FRET Studies

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Blog Post

...in energy transfer and thus, your specific FRET signal is unaffected Due to these fundamental differences...
A Primer on Optogenetics: Introduction and Opsin Delivery

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Blog Post

...of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then expression...
Trimmer Lab NeuroMab Collection

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Collection

... promoter for heavy chain expression, and an ER signal/leader sequence (L) for translocation of the heavy... promoter for light chain expression, and an ER signal/leader sequence (L) for translocation of the light...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

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Blog Post

...cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848...
Antibody Validation for Flow Cytometry

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Blog Post

...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown...

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