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We narrowed to 94 results for: Blue fluorescent Protein

Showing: 81 - 94 of 94 results

  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... express one of a few different fluorescent proteins (red, green, blue). If a researcher injects a cocktail...and neuron-specific transgene expression. Fluorescent proteins are widely used for tracing and their expression...Maday et al., 2014). It is currently unclear which proteins facilitate transport of neuronal tracing compounds...either produce a colorimetric product or are fluorescent. While dyes usually spread unidirectionally, ...biotinylated dextran amines (Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC... For predominantly retrograde tracing, albumin protein labelled with horseradish peroxidase (HRP; Kristensson...germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules such as Fluoro-Gold (Schmued and Fallon...

  3. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...expresses a unique combination of red, blue, and green fluorescent proteins in each state. The three methods...amount of information regarding which regulatory proteins, signaling molecules, mutations, and environmental...mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages...opinion, the most convenient strategy is the fluorescent reporter based method since it lends itself to...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often a FP is fused to a protein of ...enzyme cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for...bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal...elucidate the subcellular localization of your protein of interest. Find all plasmids from the Lee-Parsons...plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-50. PubMed...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...absence of a protein, determine if a protein is up or downregulated, examine a protein’s stability or ...Hjelm, 1972, Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose...support while unbound proteins are washed away.  While both Protein A and Protein G have an affinity for...compatible with either Protein A or G.  While commonly used, Protein A and Protein G are not suitable for...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the...of the target protein or interfere with protein interactions of interest. Tagged proteins are typically...modifications, or study how a target protein interacts with other proteins or nucleic acids. Read on to learn...

  6. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can find...ordered more than 20 times; red, more than 50; and blue, more than 100 times! When you search for plasmids...

  7. Optogenetics Guide

    Type
    Guide
    ...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible...Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains...photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Cryptochromes Cryptochrome 2 and CIB1 Blue-light–mediated induction of protein interactions based on Arabidopsis...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...

  8. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...molecule, the antibody binds to a protein, and then voila! Wherever your protein of interest is, a signal will...molecules to identify each protein of interest, this gel-based method identifies proteins solely by weight and... isn’t giving you enough signal to detect your protein of interest, it may be time to look at the indirect...antibodies. While a primary antibody binds to the protein of interest, a secondary antibody binds to the ...signaling molecules associated, indirectly, with each protein of interest.    Figure 1: The direct ...approach (right). Primary antibodies are represented in blue; secondary antibodies are represented in purple;... molecules are represented in light green; and proteins are represented in orange. Created with BioRender.com...

  9. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...homology dependent repair by enhanced green fluorescent protein (EGFP) reconstitution. (a) Scheme of validation...incubation, you assess the level of fluorescence in your cells under a fluorescence microscope. The higher the ...containing sgRNA sequences into HEK293T cells. The fluorescence intensity was classified into four groups (4...darker than control, 1; very dark). Brighter fluorescence indicates a more successful gRNA design. Adapted...your target is inserted, disrupting the EGFP fluorescent signal, for details on this plasmid, see Mashiko...the amount of fluorescence, the better that gRNA is at causing a double stranded break at the target site...screening as little as 8-10 pups. The level of fluorescence you saw in the validation assay should give ...

  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...method for identifying protein-protein associations. This method uses a bait protein fused to a biotin ligase...PrM N and C   Detecting protein-protein interactions with DULIP Proteins rarely act alone. Molecular...which “bait” proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with...with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based assay system...ligase to biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin ...of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins. Overall BioID2...BioID2 improves the efficiency of screening for protein–protein interactions. The Roux lab has deposited HA...

  11. Antibody Guide

    Type
    Guide
    ...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

  12. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange).... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...exciting for scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... BirA, has been used to identify and study protein-protein interactions in proximity labeling techniques...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including... Arabidopsis thaliana flowers. Image from BlueRidgeKitties. If you are working with Arabidopsis check...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Michael Ristow 14987 pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA ...Ristow 14989 pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-...Vale 15402 pBlue-hFX(AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F...Ristow 15404 pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...
Showing: 81 - 94 of 94 results