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We narrowed to 186 results for: Coli

Showing: 81 - 100 of 186 results

  1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification...Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated by guanosine...

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) Drosophila...alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...reaction (see step 3.2 ). Transform 10 μl of DH5α E. coli cells with 1 μl of reaction (from 3.2.1 - 3.2.2 ...plasmid vector. Transform the plasmid into DH5α E. coli cells and plate onto LB agar plates with the relevant.... PubMed . Transformation of plasmid DNA into E. coli using the heat shock method. Froger A, ...

  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...supplemental add-on kit and is compatible with E. coli and/or cell-free systems. Protein expression in ...al. “EcoFlex: A Multifunctional MoClo Kit for E. Coli Synthetic Biology.” ACS Synthetic Biology, vol. ...MoClo: Improved MoClo Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary... cloning that results in the creation of many E. coli constructs with greatly varied levels of expression...endeavours of unparalleled complexity in Escherichia coli, like the construction of a so-called “genomically... as a dominant-negative mutator allele of the E. coli MMR protein MutL, all under the control of the cI857...MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range of strains. Thus...but these domains often do not express well in E. coli without their partner regulatory domains. The Chodera...

  13. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011): 1151...flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation." Nature...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...phosphorylated amino acid to the genetic code of E. coli (Park et al., Science 2011). The Rinehart lab has...methodology in two differents types of bacteria (E. coli and Salmonella typhimurium) by integrating DNA sequences...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids...constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation...

  18. General Transfection

    Type
    Protocol
    ...also be propagated in an endonuclease negative E. coli strain such as NEB stable. Dilute 1:3 (µg DNA:µg...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...production of His6-MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression...cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal His and NusA fusions...
Showing: 81 - 100 of 186 results