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We narrowed to 162 results for: Cre

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Showing: 81 - 100 of 162 results

  1. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Systemic capsids packaging Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse...with appropriate cell type-specific promoters or Cre lines. Spatial transcriptomics suggests that AAV-...Huber, N., Pasca, S. P., & Gradinaru, V. (2016). Cre-dependent selection yields AAV variants for widespread...Deverman, B. E., & Gradinaru, V. (2020). Multiplexed Cre-dependent selection yields systemic AAVs for targeting...

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...CRISPRing typical mouse models, including creating Cre-dependent conditional alleles, has remained a challenge...fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff EYFP Cre AND...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT...expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...Viral Items 55636 pAAV-EF1a-Cre None Yes 55632 pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo.../Foff 2.0-EYFP Cre AND NOT Flp FRT/F5 Yes 55652 pAAV-hSyn Coff/Fon EYFP Flp AND NOT Cre No 231927 pAAV-Ef1a-Coff...pAAV-Ef1a-Coff/Fon-BFP Flp AND NOT Cre Yes 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp Yes 137133 pAAV-Ef1a-Con...

  5. The Pleiades Promoter Project

    Type
    Collection
    ...pEMS1312 cre N/A N/A pEMS1301 cre/EGFP/NLS N/A N/A pEMS1308 EGFP/cre N/A N/A pEMS1302 EGFP/cre/NLS N/A ...promoter pEMS1293 cre N/A CAG promoter pEMS1164 EGFP/cre N/A CAG promoter pEMS1114 EGFP/cre/NLS N/A CAG promoter... DBH pEMS1112 EGFP/cre/NLS Ple50 DBH pEMS1361 EGFP/NLS Ple51 DBH pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362... FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS Ple66 FEV pEMS1131 EGFP/cre/NLS Ple66 FEV pEMS1400...HAP1 pEMS1083 EGFP/cre/NLS Ple103 HAP1 pEMS1367 EGFP/NLS Ple104 HAP1 pEMS1084 EGFP/cre/NLS Ple104 HAP1 pEMS1368...Ple111 HCRT pEMS1051 cre/EGFP/NLS Ple111 HCRT pEMS1417 EGFP/NLS Ple112 HCRT pEMS1052 cre/EGFP/NLS Ple112 HCRT... HTR1A pEMS1053 cre/EGFP/NLS Ple117 HTR1A pEMS1386 EGFP/NLS Ple118 HTR1A pEMS1054 cre/EGFP/NLS Ple118 ...

  6. Retrograde AAV viral preps

    Type
    Collection
    ...AAV-pgk-Cre PGK Cre expression Recombinases Patrick Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre...Cre.SV40 CamKII Cre expression Recombinases James M. Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression... by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard...pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Bryan Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...CAG tdTomato, Cre-dependent Control Edward Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control...Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Brandon Harvey...-tagged Cre expression Recombinases James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression...

  8. Optogenetics AAV Preps

    Type
    Collection
    ...Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype...Arch GFP Cre dependent 1, 9 Edward Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato Cre dependent... EYFP Cre dependent 9 Karl Deisseroth 26966 AAV-Ef1a-DIO eNpHR 3.0-EYFP EF1a eNpHR 3.0 EYFP Cre dependent...soma-targeted) mRuby3 Cre dependent 9 Hillel Adesnik 137155 pAAV-nEF-Con/Fon-iC++-EYFP nEF iC++ EYFP Cre and Flp dependent...eOPN3 mScarlet Cre dependent 1, 5 Ofer Yizhar 139504 pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent 5...137142 pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Karl Deisseroth 137143 pAAV-nEF-Con...-nEF-Con/Foff 2.0-ChR2-mCherry nEF ChR2 mCherry Cre dependent 8 Karl Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2...

  9. Biosensor AAV Preps

    Type
    Collection
    ...Synapsin Other Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent... none Cre dependent 1 Kaspar Podgorski 135421 pAAV-syn-FLEX-axon-jYCaMP1s Syn jYCaMP1s none Cre dependent...none Cre dependent 1, 5 Loren Looger 98932 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 CAG iGluSnFr none Cre dependent...none Cre dependent 1 GENIE Project 234442 pAAV-syn-flex-iGluSnFR4f-NGR-WPRE Syn iGluSnFr4f none Cre dependent... none Cre dependent 1 Francois St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a JEDI-2P none Cre dependent...VCre-dependent Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters...pGP-AAV-syn-FLEX-jGCaMP8f-WPRE Syn jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE Project 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE...

  10. Control AAV Preps

    Type
    Collection
    ... Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive (non-cre-dependent) Clear...None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent...floxed-mCherry-WPRE-HGHpA EF1a mCherry Cre dependent 9 Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2,...EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457 pAAV-hSyn-DIO-EGFP hSyn EGFP Cre dependent 1, 2...mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent...pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Ian Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1...dTomato Cre dependent 1, 2, 5, 9, rg* Gordon Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH Syn EGFP Cre dependent...

  11. AAV Molecular Tools

    Type
    Collection
    ... a 'Cre-on' dependent manner. 1 Don Arnold 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent...99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato...FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP...45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer...Long 160857 pAAV-FLEx-ER-TurboID CMV-driven, Cre-dependent Cre-dependent expression of ER-localized TurboID...

  12. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m_3xC2-...3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_IT Isocortex 224038 pAAV-AiE0671m_3xC2-minBG-SYFP2...3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 pAAV-AiE2182m_3xC2-minBG-SYFP2...3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 pAAV-AiE2402m-minBG-SYFP2...3xC2-minBG-iCre(R297T)-BGHpA AiP14187 AiE0459m_3xC2 Cre Layer 5_ET Isocortex 224013 pAAV-AiE0674m-minBG-SYFP2...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...specific system for controlling gene expression such as Cre-lox, may be sufficient to overcome the transport ...ability to produce neuron specific transfection using Cre-lox, this new method could allow for labeling of ...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...system can be implemented by delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA to a constitutively...plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors...targeted to NeuN, plus Cre recombinase 60228 : sgRNA targeted to LacZ, plus Cre recombinase 60229 : sgRNA... cloning backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase...sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...

  15. Chemogenetics AAV Preps

    Type
    Collection
    ...dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype...Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(...Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 44362...Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 65417...kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8, rg* Bryan Roth 50454 pAAV-hSyn-DIO-HA-hM3D...hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50455 pAAV-hSyn-DIO-HA-hM4D...hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50458 pAAV-hSyn-DIO-rM3D(Gs...

  16. Hot Plasmids - October 2022

    Type
    Blog Post
    ...Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and...

  20. Recombinases AAV Preps

    Type
    Collection
    ...expression. See our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter...pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11...Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Hongkui Zeng...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato ...Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases... 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg* Esteban Engel , Alexander...none 5, PHPeB James M. Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Stanley Thomas Carmichael 214848...
Showing: 81 - 100 of 162 results