We narrowed to 877 results for: HA

...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

...Multiple Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For the purposes... design). Let's assume that your favorite vector has a relatively limited MCS (BamHI - EcoRI - SalI) and...

...protective measures for each level, as each level has different safety requirements. BSL-1 is designated...is important! Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository...

...not require specific PPE, you never know what else has been placed in the centrifuge before you and may ...the centrifuge’s internal lid on the rotor (if it has one) and then close the external lid. Adjust the ...

...Activator DREADD Bryan Roth 121539 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) Syn Activator DREADD Christopher Richie ...

...the future you can thaw a new tube that you know has only been thawed once before. Always do controls....want to see is that your vector + insert ligation has many more colonies than your vector alone ligation...

...starting this procedure, be sure that the cell pool has been sufficiently selected with the appropriate antibiotic... antibiotic so that every cell in the culture has, in theory, been transduced. There are various schools...regularly for transgene expression. This protocol has been optimized for adherent cells but can be modified...

...). Pro-Tip Add protease inhibitors to 1X if this has not already been done. We typically start with about... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. If the sample ... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. Gently transfer...

...This strategy, called genetic code expansion (GCE), has transformed synthetic biology by providing access....1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA synthetase...

...DNA that already has restriction sites on either side of it into any plasmid that has the same sites in...clonase, creating an entry clone. The entry clone now has recombined attL sites flanking your DNA fragment ...into a donor plasmid or acquire one that already has your gene in it. Then you can use bacterial recombination..., such as restriction cloning, once your project has been completed via Gateway cloning. Addgene's collection... pre-cut, linear, cloning-ready TOPO vector that has a DNA topoisomerase I covalently bound to the phosphate...base pairs. Anything shorter than 200 base pairs has the potential to be completely degraded by the 5'...repair the nicks. It is important to note that LIC has difficulty assembling DNA fragments with repetitive...

...clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 μm...such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-...

...response quickly, which has limited their use in therapeutic applications. However, this has also made them the...the human population has been exposed to at least one human AdV serotype and has developed a serotype-...immune response has become a disadvantage for the use of rAdV vectors in gene therapy but has, however, proven... digest patterns to determine that the transgene has been inserted into the adenoviral backbone plasmid...other immune-modulatory molecules. Another strategy has been to use oncolytic rAdV vectors, which are replication-competent... of spontaneous reversion to RCAs, although this has been reduced by providing the E1 gene in trans within... adenovirus (RCA) Mutated adenoviral vector that has the ability to replicate and reproduce within host...

...genome can occur, although at a low frequency which has been estimated to range from 0.01% to 3% depending... well-characterized and extensively studied, and has a broad tissue tropism. Table 1 gives a summary of...across a broad range of cell types in vivo. AAV-DJ has been optimized further for specific cell and tissue... tropism towards specific cell types of interest has been to create and test capsid libraries with random...systemic capsids. A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous...naturally cross the BBB. The Viviana Gradinaru lab has developed a number of systemic rAAV vectors that ...preps . AAV retrograde (AAVrg) Directed evolution has also been employed to engineer an rAAV serotype variant...

...beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-... MHC-G HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...MSTP084 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...

... it is possible that the difference between them has affected the accuracy of the NanoDrop. It is a good...

...include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr, ...

...expression. The Allen Institute for Brain Sciences has screened thousands of enhancers for specificity and...

...polyclonal cell population, meaning that the transgene has integrated in different locations in the various ...variety of dilutions and pick the population that has the most desirable level of expression. Over time...

...Extend DNA for 1 minute at 72°C: The Taq polymerase has an optimal temperature around 70-75°C so this step...changes during a typical PCR. The DNA polymerase has an optimum temperature around 70°C and is the molecule...

...final volume of 1 L and recheck pH to ensure that it has not drifted. Filter the solution through a 0.22 µm...incubate for 1 h at room temperature. After the BCD TFX has warmed in the bead bath, transfer the tubes to the...