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We narrowed to 387 results for: Lor

Showing: 81 - 100 of 387 results

  1. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...with spectral flow—you can run a three color panel or a 40 color panel! When preparing a spectral flow ...experiment. For very large panels (upwards of 30-40 colors), spectral flow cytometry might be the better choice... Figure 3: Similarity index across 12 different colors on a three laser spectral flow cytometer. ...from a batch that is not the same as your single color control. Reference controls: Beads or cells? Compensation...

  2. Tips for arabidopsis transformation

    Type
    Blog Post
    ...until new inflorescences begin to grow. This will also increase the number of inflorescences overall, ...mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation method. The general ...transformation, you can cut off the primary inflorescence (the flowering portion of the plant) near the...transformed Arabidopsis thaliana seedlings following floral dip transformation’, Plant Methods. BioMed Central...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... improve the efficiency of editing, including exploring new sources for the reverse transcriptase. Many...

  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...focus and exploration Researchers face a fundamental conflict. Science is about exploration, discovering...will be more competitive for fellowships. If you explore your system more, you will have a better idea of...question or are you simply describing a phenomenon? Exploration can be important, but it is equally important...foods with minimal processing, and low in empty calories will make you feel better and help maintain your...

  6. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...emission spectrum. New fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov...some types of corals and anemones are vibrantly colored. He hypothesized that GFP-like proteins could be...Discosoma sp. and Katushka/mKate from Entacmaea quadricolor. With his discoveries, another part of the fluorescent... studies in Tsien lab, covered a portion of the color spectrum that GFP and its derivatives did not previously...

  7. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... and treatment of cancer have been extensively explored. Biologists have attempted to study cancer from...ve been studying it and, now, researchers are exploring ways to bolster and modulate our immune defenses...bowel disease is associated with the onset of colorectal cancer. Strategic immune evasion mechanisms -...efficient outcomes, collaborative therapies must be explored. When attacking cancer, multiple immunotherapy...and cancer immunotherapy, and is interested in exploring new avenues in healthcare.   References 1. Vinay...

  8. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the...wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and relies ...algae host for reproduction. Snail parasite Leucochloridium is a parasitic worm that infects a snail’s ... the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723...

  9. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...and boost your productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding your...For binders of protocols and data, consider using color coded binders and tabs (for different experiments...notebooks and go wild with sticky notes and labels. Color code your Post-its and write brief descriptions ... the notebooks, so that you can easily spot the colors and text. Tip 2: Use electronic to-do lists to ...

  10. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...imaging cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012... monitor protein-protein interactions through multicolor labeling and FRET. Specialty constructs for biosensing...e.g., EGFP and YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable...at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer a comprehensive...

  11. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut Marraffini 44251 pgRNA-bacteria BBa_J23119...

  12. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University, and the Karolinska...protein CFP484. Collaborating with scientists from Florida State University, they used a mutagenesis approach... max emission at 509 nm), making it useful in multicolor labeling particularly with fluorophores that ...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology...

  13. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial...

  14. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...another gene controlling eye color) and yellow (a gene affecting the body color of the adult fly). This is...such marker is the white gene, which restores eye color to fly lines where the endogenous white gene is ...anywhere from light yellow to red. The variation in color depends on how transcriptionally active the genomic...

  15. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ... a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose...galactose and an insoluble blue pigment (4-chloro-3-brom-indigo). So far, so good, but how does this help us...β-galactosidase, and remain the lovely whitish-cream color of standard E. coli. On the other hand, intact β-galactosidase...increases pigment precipitation, enhances the blue color of negative colonies, and allows for better differentiation...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism...

  17. Open Enzyme Collection

    Type
    Collection
    ...E overhangs for Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via...

  18. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Science. Learn more by visiting the Allen Cell Explorer at allencell.org and the Allen Institute website...please visit the Cell Catalog on the Allen Cell Explorer, where you can also find the certificate of analysis...

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...plasmid with Addgene, hoping to inspire others to explore the phenomenon, improve the tool, and develop new...powerful tool, it was promising enough to keep exploring and to try developing a variant with an enhanced...York Lab operates with a strong commitment to exploration and collaboration. Andrew is a physicist with...inventors and biomedical engineers, join efforts to explore the mechanisms and develop potential applications...

  20. Control AAV Preps

    Type
    Collection
    ...Fluorophore/Tag Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags...
Showing: 81 - 100 of 387 results