We narrowed to 503 results for: RAP

...biotinylated. Read more about RapID in this blog! Find plasmids for RapID EXCELL: Proximity labeling for...APEX: engineered ascorbate peroxidase that can rapidly biotin label proteins A year after BioID became...  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo...hours to label proteins and APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic...-BioID blog post. Find plasmids for Split-BioID RaPID: RNA-protein interaction detection So far, we’ve...that can regulate multiple biological functions. RapID (RNA-protein interaction detection) uses proximity...interactions (Ramanathan et al., 2018). To develop RapID, scientists engineered a mutant BirA* from B. subtilis...

...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and ...’s size makes it difficult to deliver via gene therapy, so researchers have set their sights on another...work with dystrophin exon skipping? For such a therapy to be successful, multiple requirements must be...minimal risk of off-target editing. In order for the therapy to persist over time, stem cell editing would be...in DMD, and it’s estimated that exon skipping therapies would be applicable to 80% of DMD patients. ...characterizes additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause...represent a great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler...

...discovery/development of genetic targets and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’...researchers are now using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting... may not be the most likely candidate for gene therapy, however, because the disease affects multiple ...suggest it might work well enough to consider as a therapeutic.” There are many fundamental questions about ...mutations when using this technology. When CRISPR therapies are being given in vivo, it is difficult to use... References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology.  https://doi....1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications, Limitations, and Implications for...

...human gene therapy. Molecular Therapy: The Journal of the American Society of Gene Therapy, 14(3), 316...AAV1 was the first viral vector approved for gene therapy (Issa et al., 2023). It may be a good choice for...high transduction efficiency in multiple tissues, rapid transgene expression, and can cross blood vessel...rAAV treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes...AAV7, and AAV8 in the Mouse and Rat. Human Gene Therapy, 19(12), 1359–1368. https://doi.org/10.1089/hum...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 785. https://doi.org...in Mouse and Human Skeletal Muscle. Human Gene Therapy, 31(3–4), 233–240. https://doi.org/10.1089/hum.2019.173...

...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 1–41. https://doi.org... Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169...Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in... in the rat spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434...Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016...corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–74. https://doi.org/10.1038/s41434...

... research focuses on protein innovations for therapeutic and vaccine design. We all want tools that expand...bearing an alkyne; the two components lock together rapidly and selectively in all sorts of contexts. But the...from the regular building-blocks of living cells, rapidly forming covalent bonds to specific partners under...Adding function to My Favorite Protein (MFP) through rapid covalent coupling. Created with BioRender.com. ...intein SpyTag003/SpyCatcher003 SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded...SpyCatcher) SnoopTag/SnoopCatcher SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded...expansion DogTag/DogCatcher SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded...

...development of toolkits such as the SapTrap CRISPR/Cas Toolkit and SapTrap-SEC kit for C. elegans genome engineering...unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the need for ...rescued progeny, which makes screening extremely rapid. This stringent selection allowed me to optimize...built on this work, discovering that melting and rapidly cooling dsDNA repair templates dramatically boosted...repair template of your choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs...editing in other organisms and systems, allowing rapid progress in a wide range of fields.   Thank you...genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc...

... interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from...from the Jorgensen lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR...choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can be created in a single...single tube. You can also find additional SapTrap plasmids used for introducing point mutations that was published... Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene...PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our...

...of combining targeted molecular therapies for cancer with chemotherapies, or developing innovative mobile...clinical research to discover and test the best new therapeutics. Consultants often work with such teams to help...human resources towards the development of a new therapy based on its market need and anticipated commercial...context of significant clinical uncertainties and a rapidly evolving healthcare space. The consultants who ...at the core of the suite of benefits a given therapeutic approach brings to patients, doctors, and insurance...and the commercial applications of a particular therapy. With your scientific knowledge behind you, you...business and scientific understanding of successful therapeutics can be a great fit for former healthcare consultants...

... observations: Rapamycin is a membrane-permeable pharmacological agent that can rapidly induction heterodimerization...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP...heterodimerization of proteins that contain rapamycin-binding domains, such as FKBP and FRB. The cell does... the mitochondria (binding FRB), allowing for a rapid and inducible inactivation. Find the Robinson plasmids...approach to control for these effects, drawing on the rapidly growing field of optogenetics. Using a rational... J 2014 Aug 1;33(15):1713-26. RUSH & CRUSH – rapid & conditional gene silencing in RNAi transgenic ...CRUSH alleles off or on enables this method to rapidly address questions of tissue-specificity and cell...

...Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic applications where longer...antibodies (Ahmed et al., 2012, Bates and Power., 2019): rapid blood clearance, which is useful for imaging applications...better tissue penetration which is useful for therapeutic and imaging applications; and reduced immunogenicity... longer retention times often increase therapeutic efficacy (Ahmed et al., 2012). How do you generate ...Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic (Bates... for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments such as scFvs...Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 34:404–417 . https://doi.org/10.1089/mab.2015.0036...

...successfully helped researchers develop a variety of therapies. For example, many scientists use mice to study...the complex interactions between cancer cells, therapeutic drugs, and the rest of the body than studies ...mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at Addgene. Another...important human disease model with potential for therapeutic drug discovery as it is estimated that 75% of...they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish (Danio...elegans are small, transparent worms that have a rapid life cycle and large brood sizes. The majority of...Drosophila melanogaster and the role of the fly in therapeutic drug discovery." Pharmacological reviews 63.2...

...565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene 167-0045) Pro-...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

...more hands on While teaching courses about gene therapy and genetic manipulation, I and other professors...similar types of thinking go into developing gene therapy strategies for various diseases. That’s when I ...skills to apply those concepts and understand how therapeutic strategies work. Find Addgene Blog posts about...pick the best strategy and propose a final gene therapy approach for the disease to be openly discussed...impression was that they had clearer ideas about gene therapy in the following lessons. And let me tell you more...they had brilliant ideas on how to solve many therapeutic and pre-clinical problems! Who knows, perhaps...gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may ...

...lab from the University of Utah developed SapTrap. The SapTrap system uses golden gate assembly to produce...Ras oncogenes have been difficult to target therapeutically due in no small part to the central role Ras...restriction enzyme site and unique primer sites for more rapid screening by restriction digest and Sanger sequencing... sites, this technique also allows for easy and rapid targeting of complex elements to specific genomic...genome engineering method in bacteria that enables rapid, automated, and high-throughput genome editing is...(ssDNA) oligonucleotides (oligos), and thereby rapidly create desired allele combinations and combinatorial...et al. Metab Eng. 2012. PubMed PMID: 2232647   SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene...

...organism. These features make AAV ideal for human therapies and in vivo laboratory experiments ranging from...in the nucleus and is a good option if you need rapid transgene expression, since you don’t have to wait...transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958. Vandenberghe...adeno-associated virus manufacturing." Human gene therapy 21.10 (2010): 1251-1257. PubMed PMID: 20649475....Efficient AAV Vector Production System: Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772...the titration of AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID: 12349826. Xiong, ...Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5), 785 (2023). PubMed...

... islandicus CRISPR-Cas system. 5. Phage therapy Phage therapy uses phage to treat bacterial infections...promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas activity...bacterial pathogens possess CRISPR-Cas systems, phage therapy might not be as effective. This is where Acr proteins...enough that they could be engineered to “armor” therapeutic phages to protect them from pathogenic bacteria...activity is an important step towards CRISPR-based therapies and for more precise editing in the lab. References...Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https...

...primary human cells. Now, scientists at Beam Therapeutics have found a way to improve editing in primary... and Head of Gene Editing Technology at Beam Therapeutics, used a previously developed bacterial selection...research! Base editing in primary human cells  Cell therapy approaches can use base editors to correct mutations...efficiently makes it a promising tool for T cell therapy development.  Reducing off-target editing in ABE8s...editing specificity, for example for potential therapeutic approaches, the authors recommend mRNA delivery...off-target deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved...adenine base editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org...

...nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses... read our secondary nanobodies blog post.  A rapid selection platform for generating sybodies against...developed a robust in-vitro protocol that enables rapid selection of synthetic nanobodies, called sybodies...for further characterization and analysis. In a rapid response to the COVID-19 pandemic, the Seeger lab...lab demonstrated they could use the protocol to rapidly identify and isolate sybodies targeting the SARS-CoV...BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, ...single domain antibodies for the conformational trapping of membrane proteins. eLife 7: . https://doi.org...

... agarose addition, we found it was possible to rapidly punch out cell clones with sterile truncated pipet...from a 10 cm dish in 15- 20 minutes- a far more rapid pace than using cloning rings. A picture of a 60...only warm but not hot- and still liquid- it is rapidly poured onto the middle of the plate, where it immediately...and partially dislodged from the agarose plug by rapid pipetting, as shown in the YouTube video. Before... appear to be yielding well-spaced clones. For rapidly growing cells we sometimes use TPP peelaway T-75...drop of serum to each well in the 48-well plate; rapidly growing cells such as HEK and CHO do not seem to...technique should be useful to scientists who wish to rapidly isolate individual cell clones with different expression...