...secreted version of the protein is available – these attributes have made it popular with scientists since its...luciferase reporter vector via Gateway cloning to test for the presence of transcriptional regulatory region...

...from 40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...infection, because this setup allows for experimental replicates for each of the gRNAs. The idea was to assign...The SPARC system uses competitive recombination sites to selectively remove a stop cassette upstream of...expressed. By truncating one of the recombination sites in the construct to different lengths, the density... of Ca2+ are high in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...

... represent the official views of the National Institutes of Health....

...and terminators repressors and activators logic gates Networks and Gene Regulation Plasmids Search the...

...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...

... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

...single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These 28 plasmids...

...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...

...in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains...

...to enable identification of potential RGN target sites and to facilitate design of short oligonucleotides...

...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...

...requires delivery of hundreds of gRNAs with target sites throughout the chromosome. Mammalian ID Plasmid ...

...-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, ...neutralized. The B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization...cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission...happening.”  In their assay, the neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses...

... transcription factor and other protein binding sites. Browse, sort, or search the tables below for CRISPR...

...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

...plasmids from the Toronto, Oxford and Stockholm sites have been deposited at Addgene and are available...

...number of plates you’d like to pour. For example: Because we’d like to make 20 plates, and our plates can hold... and negative test results. Sample Data In all cases below (-) indicates that the tested strain is not... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may...Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10...colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of ...appropriate number of plates and stack them on your lab bench. Label the plates with the date and the ...remainder of the plates, pour directly from the bottle. Pro-Tips Be sure to swirl your plates after pouring...

...integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry and sfgfp...

...in a new window) Sheff MA, Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces...

...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...