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We narrowed to 888 results for: TRI

Showing: 81 - 100 of 888 results

  1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...keywords close enough... If in the past you have tried to combine multiple terms in an Addgene plasmid ...plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...Availability to Industry: Restrict search to plasmids which can be distributed to industry research scientists... GCaMP containing plasmids are available for distribution. If your experiment involves delivering a calcium...

  2. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... brain tumor biology. When not snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar...DEMONSTRATE GENOME EDITING BY THIS TOOL This post was contributed by guest blogger Pooran Dewari. Any views in ...performs Sanger sequencing on select regions of all distributed plasmids as part of quality control, but does...with the NgAgo system indicates that it is very tricky to use and fussy to optimize. One reason could ...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...enzyme." DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...like rTetR, rtTA, and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours),...Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral ...Tet-Off and additional plasmids. tetR-KRAB TRE Patrick Aebischer , Didier Trono 41393 pCW57.1 Lentiviral...

  4. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Michael Davidson from Florida State University has contributed their comprehensive collection of ORFs tagged...found below. In addition, the Davidson lab has contributed the corresponding empty backbone with different...

  5. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...plasmids available to scientists in academic and industrial organizations. For more information on ordering... a for-profit entity, please see our Plasmid Distribution to Industry page. These plasmids have not been...

  6. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core... have the right to use the original plasmid. Distribution of the original plasmid is important because...

  8. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...cells to let the virions out. We recommend using a Tris-based lysis buffer and sonication.  The obtained...density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin,..., and the two helper plasmids, commonly called “triple plasmid transfection” (Figure 1).    Figure 1:...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  9. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ... be a shortage of amusing reports where someone tries to “optimize” a particular standard lab protocol...technical knowledge and judgement. For particularly tricky cases, team discussions can often lead to otherwise...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine is very effective at disrupting...and blue triangles. 2) An antibody immobilized on a bead binds specifically to the blue triangle protein...not the other. 3) The beads are collected by centrifugation or a magnet. 4) Unbound proteins are washed...target protein. The beads can be collected by centrifugation (agarose beads) or a magnet (magnetic beads...particularly advantageous because they do not require centrifugation between washes which can cause loss of beads...containing non-ionic detergents, such as NP-40 or triton-X, to preserve native protein conformations and...proteins, including those in the nucleus, more stringent buffers may be needed. Since many factors, including...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    ...If you’ve heard of containerization, but haven’t tried it or are confused by the concept, don’t worry–you... developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting, or sequence...first n items. To be sure, we went through much trial and error to learn the scripting language, but our...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

  12. Lab to Office Culture Shock

    Type
    Blog Post
    ...     Over the last decade, my peers and I have tried to move away from calling non-academic careers and...hands-on lab opportunities. You can get your hands in nitrile gloves again! The opportunities are there once ...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...populations can arise very quickly following the tried and true process of evolution. Watch this video ...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in...resistant to antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally...resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species’ resistance...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...be unharmed by the compound it produces (think intrinsic resistance), but surrounding microbes would be...resistance genes survive and multiply. Factors contributing to the development of resistance include (Figure...

  14. AAV for Neuronal Tracing

    Type
    Collection
    ...retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

  15. Antibody Plasmid Collection

    Type
    Collection
    ...Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies made from...neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids...

  16. mTOR Pathway

    Type
    Collection
    ...mTORC1 integrates these signals to determine the nutritional state of the cell and regulates the switch from...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including...

  17. Arf GTPase Family

    Type
    Collection
    ...GTPase, and GAP set is referred to as a minimal triad, with each component involved in GTPase signaling..., Gateway GTPase Sar1b 51128 198 Gateway GTPase Trim23 373 574 Browse ARF GAP and GEF Plasmids by Gene...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...zebrafish ( Danio rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater...Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines from European researchers...

  19. TALEN Plasmids and Kits

    Type
    Collection
    ... Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN...cell lines. Features include (i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden ...

  20. Control AAV Preps

    Type
    Collection
    ...IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Marcella Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry Constitutive...pAAV-Ef1a-fDIO-tdTomato EF1a tdTomato Flp dependent 1 Patricia Jensen 99133 pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen...
Showing: 81 - 100 of 888 results