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We narrowed to 401 results for: caps

Showing: 81 - 100 of 401 results

  1. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...process of anchoring an antibody, often called the capture antibody, to agarose or magnetic beads in a way...immunoglobulin, leaving the antigen binding sites free to capture the target protein. The beads can be collected ..., and perform the IP at 4oC. When choosing the capture antibody for IP, be sure to choose one that recognizes...proteins that may interact nonspecifically with the capture antibody or Protein A/G. To preclear, incubate ...nonspecific antibody from the same host species as the capture antibody. This will deplete the nonspecific proteins... the target protein in the sample binds to the capture antibody and is immobilized. The ideal antibody...antibody concentration to use will vary between capture antibodies and can be determined through titration. ...

  2. Rett Syndrome

    Type
    Collection
    ... MECP2 null alleles or common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats...

  3. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...relative to the total number of viral capsids (which can include empty capsids that are devoid of a viral genome... viral particles. Ratio of full to empty viral capsids: the ratio of genome-containing viral particles...genome). Empty viral capsids can be formed during viral production depending on the  experimental conditions...One study showed that approximately 50% of viral capsids were genome-containing (“full”) (Zeltner et al....1999). The percentage of genome-containing viral capsids is typically quantified by electron microscopy ...we are pleased to report that over 95% of viral capsids in representative viral vector preparations are...purified viral vector solution (which contains viral capsid proteins) as opposed to purified DNA. This may ...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...and invade multiple organs, and most importantly escape the immune mechanisms of the host. The cause, manifestation...drug resistance in bacteria, the tumor cells that escape the elimination stage acquire genetic alterations...antigen production to evade the immune system. 3. Escape - Tumor cells that survive this phase are able ...outsmart the host immune mechanisms altogether and escape future immune surveillance. Dormancy of certain...interactions with the immune system, tumors “learn” how to escape it. In this process, cancer cells develop and thrive...Evading Recognition Tumor cells over-express CD47 to escape recognition by circulating macrophages. Tumor cells...metastasis, block the cell-surface proteins that help escape immuno-surveillance, and curb cytokine and chemokine...

  5. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ... users coat a multiwell plate with a capture reagent. The capture reagent may be an antigen or an antibody...on how the assay is set up. Then they block the capture reagent to prevent non-specific interactions. Next...ELISA the multiwell plate is first coated with a capture antibody that binds to the antigen in a sample ...ELISA, users first coat a multiwell plate with a capture antibody and then add their antigen-containing ...antigen-containing sample. The target antigen binds to the capture antibody and a series of wash steps removes non-target....  Importantly, the detection antibody and the capture antibody must bind to distinct, non-overlapping...they require two independent antibodies for the capture and detection steps, which increases the specificity...

  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...developing new capsids through direct evolution, capsid shuffling, and peptide displays. These capsids will be...slight variations in their viral capsids. Novel forms of AAV capsids are discovered frequently and recent...activation of innate immunity. AAV vectors are also capable of infecting a wide range of cell types including...enabled us to generate new and refined forms of capsids with improved specificity. AAV vectors are able...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified...using AAV is the limited packaging capacity. Lentiviruses have capacities of 8–12 kb, while AAV only has ...libraries available at Addgene. The large packaging capacity and stable expression of lentiviral vectors make... AAV, with the genome from one serotype and the capsid of another. Researchers have used these psuedotypes...Interest in interrogating and mapping the genetic landscape of tissue-specific diseases has grown exponentially...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...antibodies, Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] and Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86], target...target distinct epitopes on the SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins...Figure 3: HeLa cells transduced with SARS-CoV-2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane...) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies #211756 (left panel) or #211757..., et al. (2021). Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody. Virology. 558,...-strand AAV (ssAAV). To maintain the packaging capacity and improve the specificity of ssAAV, a targeting...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...(Cas) genes as well as non-coding RNA elements capable of programming the specificity of the CRISPR-mediated...

  10. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...intuitive and I was making figures quickly. Inkscape Inkscape is the most similar to Adobe Illustrator out...and like Vectr, Inkscape uses vector graphics. There’s a steep learning curve for Inkscape, but there are... desktop use for Windows, Mac OS X, and Linux. Inkscape is versatile and has a wide selection of tools...lots of resources for learning the software. The Inkscape website has many tutorials and Lynda has a tutorial...

  11. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ... compared to the wild-type, escaped neutralization. “That degree of escape from a very small number of...luminescence.     B.1.351 variant neutralization escape The lab saw that the B.1.351 variant was very resistant...what was surprising was the extent to which the escape appeared to be happening.”  In their assay, the...-YS, Lessells RJ, de Oliveira T, Sigal A (2021) Escape of SARS-CoV-2 501Y.V2 from neutralization by convalescent..., Balazs AB (2021) Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity...

  12. Hot Plasmids - May 2022

    Type
    Blog Post
    ... AAV capsids (left) and transduction patterns (right).  Both AAV-PHP.eB and the AAV.CAP-B capsids are ...positive and negative selective pressures for novel capsids. In a continuation of that work, they have found...is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given that the liver is an ... derived from AAV9.  Of those shown here, only AAV.CAP-B10 causes brain wide expression, with no liver...

  13. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...to the type of capsid (outer protein shell) used to package an rAAV vector; these capsids allow the virus...AAV components      Cargo capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your... virus, packaged in a non-enveloped icosahedral capsid, that can be used to express genes of interest ...plasmid and individually packaged into the AAV capsids. As a genome conversion in the transduced cell ...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...56,762 (Set A) 56,476 (Set B) Broad GPP activation Caprano p65-HSF 92383 (Set A) 92384 (Set B) Activation ...

  15. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...select novel AAV capsid variants could unintentionally limit the utility of candidate capsids.  Are you interested...small randomized peptide loop inserted in the AAV9 capsid. This mutant library was then screened in vivo ...version of LY6A, but a mutation in PHP.B’s unique capsid loop disrupted binding. Collectively these results...LY6A, a novel cellular receptor for AAV-PHP. B capsids." bioRxiv (2019): 538421. If you’re interested ...administration of the adeno-associated virus-PHP. B capsid fails to upregulate transduction efficiency in ...

  16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... determine if CRISPR/Cas9 escape occurs in an animal model. If these escape mutations are frequent, the...their results, Wang et al. showed that HIV-1 could escape from CRISPR/Cas9-induced modification targeting...either the LTRs or essential genes. Many of these escape mutations were located near the Cas9 cut site, ...1 infection, which would make it easier for an “escape” virus to propagate and infect adjacent cells. ... Inhibit HIV-1 Replication and Accelerate Viral Escape. Cell Rep. 2016 Apr 5. PubMed PMID: 27068471. Ramanan...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... for the identification of plasmid multimers. Capillary Gel Electrophoresis (CGE) analysis Another technique...technique that can detect multimers is capillary gel electrophoresis (CGE), which has been used in plasmid...Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med. 2020;20(10)...PMC4480603. Holovics HJ, He Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with laser-induced fluorescence...fluorescence of plasmid DNA in untreated capillary. Electrophoresis. 2010 Jul;31(14):2436-41. doi: 10.1002/...study and topology analysis of plasmid DNA by capillary gel electrophoresis. BioProcess Int. 2006 Sep;...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...interactions , protein dimerization , endosomal escape of cell-penetrating peptides , and more. nLuc/cLuc...

  20. Antibodies 101: Validation

    Type
    Blog Post
    ...your experiments. Capture MS Strategies This approach uses immunoprecipitation to capture proteins from a...and then uses mass spectrometry to identify the captured proteins. If the antibody is specific, then you...approach is ideal for validating antibodies for immunocapture applications and is amenable to high-throughput...to validate that your antibody is specifically capturing your RNA-binding protein of interest. So you ...immunoprecipitation with your antibody and send the captured proteins off for mass spec analysis (above). When...
Showing: 81 - 100 of 401 results