Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 286 results for: lum

Showing: 81 - 100 of 286 results

  1. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...  Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends ... through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain cell...

  2. Lentiviral Prep Service

    Type
    Collection
    ... in solution that are suitable for in vivo use. Volume and minimum titers are listed on each item's material...

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...injections are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis et...parentheses in the"Mouse Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-...transducing the macaque brain, except for the cerebellum, brain stem, and spinal cord. All PALs, including...compared to AAV9 in the cerebrum, but not the cerebellum, in macaques. It also effectively transduces ...

  7. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment... the equation is labeled with its corresponding column in the calculator spreadsheet: We hope that this...

  8. COVID-19 Resources

    Type
    Collection
    ...nonprofits, and this is denoted in the Industry column below. For more information on ordering from a ...

  9. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...Red2Green, a program that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack...this material to produce various grades of plastic lumber and distribute it to industrial and residential...

  10. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...

  15. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to ...electrical activity through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used...

  16. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... The amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...

  18. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

  19. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... particles to your solution while still using a volume that’s compatible with your experimental setup.... the filter - When using a concentration filter column, make sure to rinse off the surface of the filter...

  20. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...
Showing: 81 - 100 of 286 results