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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 287 results for: lum

Showing: 81 - 100 of 287 results

  1. Plasmid Collections

    Type
    Collection
    ... Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics...

  3. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment... the equation is labeled with its corresponding column in the calculator spreadsheet: We hope that this...

  4. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...Red2Green, a program that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack...this material to produce various grades of plastic lumber and distribute it to industrial and residential...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...

  8. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... The amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...

  11. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to ...electrical activity through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used...

  13. Lentiviral Prep Service

    Type
    Collection
    ... in solution that are suitable for in vivo use. Volume and minimum titers are listed on each item's material...

  14. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... particles to your solution while still using a volume that’s compatible with your experimental setup.... the filter - When using a concentration filter column, make sure to rinse off the surface of the filter...

  15. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...

  16. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

  17. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists, while neuroscientists... Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a FRET Based Biosensor Fluorescent...

  18. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them...

  20. Antibodies 101: Conjugation

    Type
    Blog Post
    ...either through a chromogenic or chemiluminescent reaction. Chemiluminescence, which is light produced by ...
Showing: 81 - 100 of 287 results