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We narrowed to 252 results for: p

Showing: 81 - 100 of 252 results
  1. Viral Production

    Type
    Collection
    ... element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization...
  2. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...J. E., Dunn, B. M., Speicher, D. W., & Wingfield, P. T. (Eds.). (2001). Current Protocols in Protein Science...Maltose-Binding Protein as a Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols...
  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ....2019.01.003  Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). An In Vivo Covalent TMP-Tag...better-dyeing-through-chemistry-small-molecule-fluorophores  Los, G. V., Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N....M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins, A., Klaubert, D...10.1021/cb800025k  Miller, L. W., Cai, Y., Sheetz, M. P., & Cornish, V. W. (2005). In vivo protein labeling...
  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR...References Chew WL, Tabebordbar M, Cheng JKW, Mali P, Wu EY, Ng AHM, Zhu K, Wagers AJ, Church GM (2016)...
  5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...PubMed Central PMCID: PMC2593125. 2. Buchholz, F, P O Angrand, and A. Francis Stewart. 1998. “Improved...Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase of the Saccharomyces...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...
  6. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...guides are used to call hits from the growth screen, p-values were less significant than when non-targeting...non-targeting guides are used as controls. Less significant p-values lead to more false-negatives results (i.e. ... library resulted in both increased and decreased p-values of known ricin regulation genes compared to...
  7. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti ...doi.org/10.1371/journal.pone.0158294 Chari R, Mali P, Moosburner M, Church GM (2015) Unraveling CRISPR-...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan...
  8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...and resources References Banasik, M. B., & McCray, P. B., Jr (2010). Integrase-defective lentiviral vectors...Bayford, J., Brown, L., Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar,... Central PMCID: PMC189872 Schröder, A. R., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman, F....
  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules...Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck,.... 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted...
  10. Plant Plasmids and Resources

    Type
    Collection
    ...Physcomitrella patens System for genome editing of the moss P. patens . CRISPR Magdalena Bezanilla 1000000270 ENABLE...
  11. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation... https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl, R., & Cooley, R. (2022). Site-specific Incorporation...
  12. Antibodies 101: Isotypes

    Type
    Blog Post
    ...References and resources References Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System...www.ncbi.nlm.nih.gov/books/NBK27106/  Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System...
  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...organism. Precursor, or pre-tRNAs are cleaved by RNase P and Z in eukaryotes (E in bacteria), and only a few...cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. doi: ...
  14. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...s41467-019-12339-7 Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M, Zoldoš V (2016) Repurposing...Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing in vivo by programmable...
Showing: 81 - 100 of 252 results