We narrowed to 241 results for: p
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TypeBlog Post...PubMed Central PMCID: PMC2593125. 2. Buchholz, F, P O Angrand, and A. Francis Stewart. 1998. “Improved...Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase of the Saccharomyces...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...
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CRISPR Activation: A Practical Guide
TypeBlog Post...Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti ...doi.org/10.1371/journal.pone.0158294 Chari R, Mali P, Moosburner M, Church GM (2015) Unraveling CRISPR-...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan... -
Lentivirus ddPCR Titration
TypeProtocol...Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post... J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...and resources References Banasik, M. B., & McCray, P. B., Jr (2010). Integrase-defective lentiviral vectors...Bayford, J., Brown, L., Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar,... Central PMCID: PMC189872 Schröder, A. R., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman, F.... -
Plasmids 101: Biotinylation
TypeBlog Post... PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules...Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck,.... 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...hypothesis testing statistical tools that generate a p-value. As an example, if you hypothesize that growth...significant result that may be the result of noise, p-hacking, or unclear methods. Many thanks to our guest... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post... for the wild-type. However, variants such as the P.1 (Gamma variant) and the B.1.351 (Beta variant) were...lowest levels of cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation... https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl, R., & Cooley, R. (2022). Site-specific Incorporation... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...stays open for a longer duration upon activation. p-mcherry-C1-iC++_CA - A slow cycling (open for minutes...20026026. PubMed Central PMCID: PMC2823934. Pai, Vaibhav P., et al. "Transmembrane voltage potential controls... -
Antibodies 101: Isotypes
TypeBlog Post...References and resources References Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System...www.ncbi.nlm.nih.gov/books/NBK27106/ Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post... 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein instability following... B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M, Cleaver BD, Clément N, Byrne ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...organism. Precursor, or pre-tRNAs are cleaved by RNase P and Z in eukaryotes (E in bacteria), and only a few...cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. doi: ... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...s41467-019-12339-7 Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M, Zoldoš V (2016) Repurposing...Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing in vivo by programmable... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens...doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...10.1038/nbt.2508 Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington...10.1128/mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post..., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang...//doi.org/10.1128/mcb.18.1.93 Ran, F. A., Hsu, P. D., Lin, C., Gootenberg, J. S., Konermann, S., Trevino...doi.org/10.1016/j.cell.2013.08.021 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang...., Meng, X., Urnov, F. D., Rebar, E. J., Gregory, P. D., Zhang, H. S., & Jaenisch, R. (2011). Generation... -
RNA Extraction Without A Kit
TypeBlog Post.... https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987) Single-Step Method of RNA Isolation.../doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...genes that affect autophagy (ATG) in yeast. Atg8p (“p” stands for protein) associates with autophagosomal...PMC2852113. 4. Klionsky, D. J., Cuervo, A. M., & Seglen, P. O. (2007). Methods for Monitoring Autophagy from ... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post... J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post... immunity to viral invasion. His lab is studying P. furiosus, a hyperthermophile that exists in sea vents...regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against both DNA and...