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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 239 results for: p

Showing: 81 - 100 of 239 results

  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules...Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck,.... 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted...

  3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...and resources References Banasik, M. B., & McCray, P. B., Jr (2010). Integrase-defective lentiviral vectors...Bayford, J., Brown, L., Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar,... Central PMCID: PMC189872 Schröder, A. R., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman, F....

  8. Antibodies 101: Isotypes

    Type
    Blog Post
    ...References and resources References Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System...www.ncbi.nlm.nih.gov/books/NBK27106/  Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation... https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl, R., & Cooley, R. (2022). Site-specific Incorporation...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...organism. Precursor, or pre-tRNAs are cleaved by RNase P and Z in eukaryotes (E in bacteria), and only a few...cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. doi: ...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...s41467-019-12339-7 Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M, Zoldoš V (2016) Repurposing...Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing in vivo by programmable...

  12. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens...doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...10.1038/nbt.2508 Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington...10.1128/mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ..., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang...//doi.org/10.1128/mcb.18.1.93  Ran, F. A., Hsu, P. D., Lin, C., Gootenberg, J. S., Konermann, S., Trevino...doi.org/10.1016/j.cell.2013.08.021  Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang...., Meng, X., Urnov, F. D., Rebar, E. J., Gregory, P. D., Zhang, H. S., & Jaenisch, R. (2011). Generation...

  14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...genes that affect autophagy (ATG) in yeast. Atg8p (“p” stands for protein) associates with autophagosomal...PMC2852113. 4. Klionsky, D. J., Cuervo, A. M., & Seglen, P. O. (2007). Methods for Monitoring Autophagy from ...

  15. RNA Extraction Without A Kit

    Type
    Blog Post
    .... https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987) Single-Step Method of RNA Isolation.../doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Biomedical Optics, Feburary 2005, Vol. 10 No. 1, p. 14003 Chu et al. : Nature Methods, May 2014 Vol. ... : Nature Communications, May 2014 Vol. 15 No. 5, p. 3626 Do you have suggestions for other plasmids that...

  18. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile that exists in sea vents...regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against both DNA and...

  20. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine...383. PubMed PMID: 12605205. 13. Yan, Qi, and Marcel P. Bruchez. "Advances in chemical labeling of proteins...
Showing: 81 - 100 of 239 results