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We narrowed to 133 results for: primer

Showing: 81 - 100 of 133 results

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of CRISPR/Cas9...

  6. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., 2012 ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT...10 μL 1,000 μL 100 μM Forward Primer 0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water...GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free... SYBR master mix, then the forward and reverse primers. Vortex briefly, immediately before use. Use a ... of ~70–75 °C usually indicates the presence of primer dimers which can increase background signal and...

  15. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

  19. Molecular Biology Reference

    Type
    Guide
    ... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...enzyme, the template DNA to be copied, and a primer. A primer is a small piece of DNA, approximately 18-...select or sort the cells by visualization or FACS). Primer Binding Site A short single-stranded DNA sequence...amplification or DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert...know some of its sequence to design a effective primer. Sanger sequencing chromatogram Sanger sequencing...replication, the Sanger sequencing reaction begins when a primer binds to its complementary DNA and the DNA polymerase...approximately 500-1000 bases downstream of the known primer region with very few errors making it an efficient...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...expressed is amplified using a gene-specific forward primer containing the desired restriction site (the MCS...has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...if a small contaminating PCR product formed from primer dimers and containing the missing codons from the...resistance gene is inserted. It is important that primers be designed to minimize the possibility of dimer...
Showing: 81 - 100 of 133 results