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Addgene
Showing: 81 - 100 of 421 results
  1. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...and their successes. Although all mentorship relationships are valuable and highly recommended, a mentor...mentor creates a closer and more approachable relationship for the mentee, where (s)he feels truly understood..., mentors and mentees often developed close relationships with a level of sympathy that is difficult to...confidence. These improvements, combined with the relationships fostered through the IMP have motivated many...-Harvard Medical School, where she studies age-related macular degeneration. She is also a blogger who...
  2. Antibodies 101: Validation

    Type
    Blog Post
    ...your target then you would expect to see good correlation between the levels of target detected in both...both methods. These approaches have relatively low sensitivity, which can be problematic if you are trying...also influence antibody binding by altering the relative concentration of potential antibody binders and... targeting a different epitope, and look for correlation between the results. Using antibodies against...cytometry using both antibodies, as well as a third unrelated antibody as a negative control (A). The two antibodies...
  3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...particles or relatively short expression times, where as others, such as hSynapsin, are relatively weak and...sites and the open reading frame is inverted in relation to the promoter, hence the name, double-floxed...homologous pairs, and that the degree of homology is correlated with the level of spontaneous recombination. ...this study were packaged at Addgene and had a relatively low recombination rate (0.1% and 0.13% respectively...
  4. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...brightness for a few commonly used fluorophores in relation to the laser type. Table adapted from: https:/...are absorbed and then emitted, which creates a relatively stronger—or brighter—signal.   Figure ...use brighter fluorophores for markers that are relatively lower in abundance. In contrast, when you expect...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how... into different levels of brightness and their relation to the fluorophore's excitation efficiency, and...
  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...
  6. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...slight or large variations depending on how closely related these species are. If a gene was moved exactly ...exactly as is from one organism to a distantly related organism, the gene may not express well because of these...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then...growing trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the genes deposited...
  7. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    ...seeds, DNA and other resources for Arabidopsis and related species. Located at The Ohio State University in...member of the Brassicaceae family, Arabidopsis is related to a variety of food crops such as broccoli, cabbage...utility to science, and its well-known edible relatives, it is fair to say that most people do not recognize...Arabidopsis is small, has a short life cycle, and is relatively easy to grow, it is an ideal organism for teachers...
  8. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ... of the time because of relationships. It’s much harder to build relationships when you are more desperate...experiences to share. The conversation builds a relationship for the future and the 1:1 format is good for...you will make good progress on both building relationships and thinking about future career options. 5....
  9. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...using simple web forms) while also maintaining a relatively strict database design (schema).  Registered ...allows for visual comparison of FPs and how they relate to one another. .  In addition, the FPbase Fluorescence... page with all the information in the database relating to that specific protein (for instance: EGFP)....retrieve all of the information in the database relating to that set of proteins.  For computer-savvy users...
  10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...participants in skills useful for future mentoring relationships.   If you would like to start a group mentoring...for the time commitment of a formal mentoring relationship or there aren’t enough senior mentors available...ideas on what to do once you are in a mentoring relationship.  Celebrate your successes and support each... come. Are you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring...
  11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ... Sensitivity Cas9 sensitivity is often closely related to specificity. Sensitivity is a measure of the...the enzymes showed decreased off-target activity relative to Cas9. Since then, the Joung and Jennifer Doudna...showed decreased or similar off-target activity, relative to eSpCas9 and SpCas9-HF across a number of different...research due to its small size and specificity relative to Cas9. As scientists continue to characterize...
  12. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...is the question. When presenting on building relationships (also known as “networking”), one of the most...networking to start, build, and track professional relationships. Here are my seven best tips beyond the basics...on LinkedIn!” resulting in just a little more relationship. Pro tip: Once you are connected with someone...connection requests LinkedIn is a place to build relationships. If you don’t add a personal note, you waste...
  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...highlight four tools for finding journal articles related to your actual interests while also staying on ...these types of accounts can help you find papers related to a particular topic or that were published by...tweet about papers too. You can follow journals related to your field of research or larger journals that... on a paper in your feed you can see suggested related researchers, journals, and concepts to help you...
  14. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... one-copy ‘housekeeping’ gene), and quantified relative to a 'normal' sample with known copy number set...nice thing about qPCR is that since it measures relative quantification - that is, since you’re looking...looking at the amount of DNA in your sample relative to your normal control - you can set up your calculations...provides absolute quantitative data, instead of relative quantification.  Even though no one option is ...
  15. Transferable Skills: Negotiation

    Type
    Blog Post
    ... the ultimate resolution can involve ideas not related to the actual data collection task but still be...negotiation skills will help you strengthen relationships with your colleagues and supervisors and lead...negotiation skills are key to maintaining and growing relationships with your colleagues within and outside of your... what you want and can also strengthen your relationships with others who know that working with you will...
  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...attending the session, and what is their background relative to your research? Tailor your language to your... you may receive. People often ask about the relationship of your work to recently published papers or...audience just that. Explain what you do know about a related topic, and let them know while you appreciate the...collaborate with a group who presented on a topic related to your research? Find a way to get in contact ...
  17. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... If you are miserable at home or work, your relationships will suffer in both the short run and the long...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The... make your life easier and to have more time to relax and be with friends and family. #7 Thou shalt create...swim lesson missed can mean a world of mental relaxation. And, I’m not sure I should put this in print...
  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...al. found that translation efficiency is well correlated with codon bias in both E. coli and S. cerevisiae...with specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions...theory, this is not as easy as it sounds. Even for relatively short peptides, there can be many possible ways...non-exhaustive list includes: Codon abundance relative to cognate tRNA abundance Repetitive sequences...
  19. Viral Production at Addgene

    Type
    Blog Post
    ...of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins...SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed...Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total protein present in the sample. ...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale bar...
  20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...editing - enabling a ~100-fold reduction in cost relative to NGS-based amplicon sequencing. To use the tool...different types of edits that are present and their relative abundances.   The ICE tool also provides a Knockout...during generation of the ICE Score, the Pearson correlation coefficient (r) is also computed and reported...sequences present in your edited population and their relative representation in the edited pool. The black vertical...
Showing: 81 - 100 of 421 results