...practically this isolation is only feasible for relatively short DNA molecules. In order to isolate a particular..., and thus the plasmid exists in a symbiotic relationship with the host cell. Like the bacterial chromosomal...most plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs) to enable easy...Because bacteria are easy to grow in a lab, divide relatively quickly, and exhibit exponential growth rates...recommend checking out these two Addgene blog posts relating to common E. coli lab strains and E. coli strains...specialized for protein expression for additional strain-related information and a more extensive strain list. Strain...endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega e14-(McrA-) recA1 endA1...

...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...instead of modified antibodies and are not closely related to antibodies. Monobodies - Monobodies are synthetic...colored product that accumulates over time which is relative to the amount of enzyme present in each well. ... assays, protein amount can be determined by correlating the amount of color in a well to a standard curve... categories: Quantification - used to quantify relative amounts of protein. Includes: Western blot ELISA... western blots or ELISAs, are used to quantify relative amounts of protein. Western blot Western blots...proteins separated by size on an SDS-PAGE gel. Though relative quantification can be done, western blots primarily...

...efficiency of Cas9 cleavage is relatively high and the efficiency of HDR is relatively low, a large portion of...recognizes the NG PAM, has increased activity in vitro relative to other Cas9 endonucleases. Read more about Cas9...SpCas9 for specific applications. For example, the relatively large size of SpCas9 (∼4 kb coding sequence) ...

...any of our eBooks to learn about popular topics related to our collections. Titles include: Plasmids 101...Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics include: Genome...

...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...

...start learning about various scientific concepts related to our plasmid collection. Find links to the guides...

...non-enveloped double-stranded DNA viruses. They are not related; however, AAV requires the presence of adenoviral...

..., Jin J (2015). The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...

... to Top Glossary Plasmid Type Element Delivery relative to transgene Purpose Envelope VSVG in trans Vesicular...