We narrowed to 14 results for: rela

1. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ... A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B...terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids available from ... have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene recommends that you select...

2. Video Library

   Type

   Protocol

   ... opens in a new window) Video Link Description Related Page Making LB Agar Plates Create plates to culture... opens in a new window) Video Link Description Related Page MTA Guide - Requests Your guide to the MTA...

3. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and to minimize well-...following: Standard curve: R 2 (coefficient of correlation) ~ 1.0, E (efficiency of PCR) ~100% (90%–110%...

4. Lentivirus ddPCR Titration

   Type

   Protocol

   ... across the dilutions. RPP30 copies should be relatively constant across samples. In the RRE example below...concentration of RPP30 positive droplets stays relatively even across samples (green). To increase the ...

5. Protocol - How to Design Primers

   Type

   Protocol

   ...in size. The structure of the primer should be relatively simple and contain no internal secondary structure...

6. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ... Let's assume that your favorite vector has a relatively limited MCS (BamHI - EcoRI - SalI) and you want...

7. Water Bath Protocol

   Type

   Protocol

   ... your experiment. Water baths tend to heat up relatively slowly. Place the water bath cover on the top...

8. Centrifugation

   Type

   Protocol

   ...protocol. Pro-Tip Spin speed is often given as Relative Centrifugal Force (RCF) or Revolutions Per Minute...

9. Protocol - Bacterial Transformation

   Type

   Protocol

   ...also make their own competent cells. This is a relatively simple procedure and is useful for performing...

10. Pipetting Protocol

    Type

    Protocol

    ...be in the liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid...

11. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...be 10 µg/mL. Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction...

12. Coomassie Purity Stain of Recombinant Antibodies

    Type

    Protocol

    ...) or a similar photo software to determine the relative intensity of the protein bands to the overall ...

13. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ...hemocytometer or other cell counter. Pro-Tip Prepare a relatively dilute cell solution (<10 6 cells/mL) before ...

14. CRISPR Library Amplification

    Type

    Protocol

    ...digest. Recombination is thought to be in part related to selective pressure. These recombined plasmids...