...real Addgenies! Protocols Video Link Description Related Page Making LB Agar Plates Create plates to culture... Protocol How-To Videos Video Link Description Related Page MTA Guide - Requests Your guide to the MTA...

... A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B...terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids available from ... have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene recommends that you select...

...reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and to minimize well-...following: Standard curve: R 2 (coefficient of correlation) ~ 1.0, E (efficiency of PCR) ~100% (90%-110%...

... across the dilutions. RPP30 copies should be relatively constant across samples. In the RRE example below...concentration of RPP30 positive droplets stays relatively even across samples (green). To increase the ...

...in size. The structure of the primer should be relatively simple and contain no internal secondary structure...

... Let's assume that your favorite vector has a relatively limited MCS (BamHI - EcoRI - SalI) and you want...

... your experiment. Water baths tend to heat up relatively slowly. Place the water bath cover on the top...

...protocol. *Pro-Tip* Spin speed is often given as Relative Centrifugal Force (RCF) or Revolutions Per Minute...

...also make their own competent cells. This is a relatively simple procedure and is useful for performing...

... in the liquid. *Pro-Tip* If pipetting from a relatively large container holding a small volume of liquid...

...10 µg/mL. *Pro-Tip* Transducing too many cells relative to the number of virus particles reduces the transduction...

...ImageJ or a similar photo software to determine the relative intensity of the protein bands to the overall ...

...hemocytometer or other cell counter. *Pro-Tip* Prepare a relatively dilute cell solution (<10 6 cells/mL) before ...

...digest. Recombination is thought to be in part related to selective pressure. These recombined plasmids...