We narrowed to 1,082 results for: ica;

1. Some Like it Hot: Thermostable GeoCas9

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   Blog Post

   ...could also be used with in vitro molecular bio applications that require high temperature reaction conditions...

2. Management for Scientists: Giving Feedback

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   ... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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   ...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...

4. New and Upcoming Viral Vectors - December 2019

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   ...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

5. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   ...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...

6. Components of CRISPR/Cas9

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   ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   ... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...

9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   ... of the entire plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing...

10. New and Upcoming Viral Vectors - May 2020

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    Blog Post

    ...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

11. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

12. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...have good antibodies. The results look almost identical. Addgene: What advantages does your system have...

13. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    .... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

14. Transferable Skills: Negotiation

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    ...contracts behind closed doors. Fig. 1: The mythical land of negotiations.    You negotiate everyday...

15. Plasmids 101: Multicistronic Vectors

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    ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

16. Plasmids 101: Colony PCR

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    ...-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine ...

17. Negotiating Work and Life: How to Find the Joy

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    ...help you do your job better. #8 Thou shalt enthusiastically foster independence Your partner can make ...

18. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...

19. Plasmids 101: Codon usage bias

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    Blog Post

    ...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...

20. Filming Science Videos in the Age of Social Distancing

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    Blog Post

    ...focus or zoom on a camera lens without having to physically rotate the rings around the lens. It allowed ...